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Problem 8.10a
A DNA microarray consists of __________ .
a. a series of clones containing the entire genome of a microbe
b. recombinant microbial cells
c. restriction enzyme fragments of DNA molecules
d. single-stranded DNA localized on a substrate
Problem 8.1a
Indicate which of the following are true and which are false. Rewrite any false statements to make them true by changing the underlined words.
__________ Restriction enzymes inhibit the movement of DNA at specific sites.
Problem 8.1a
Describe three artificial methods of introducing DNA into cells.
Problem 8.1a
Label the reagents and steps of PCR on the following figure. Indicate the temperature of the reaction at each numbered step.
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Problem 8.2a
Indicate which of the following are true and which are false. Rewrite any false statements to make them true by changing the underlined words.
__________ Restriction enzymes act at specific nucleotide sequences within a double-stranded DNA molecule.
Problem 8.2a
Why is cloning a practical technique for medical researchers?
Problem 8.3a
Indicate which of the following are true and which are false. Rewrite any false statements to make them true by changing the underlined words.
__________ A thermocycler separates molecules based on their size, shape, and electrical charge.
Problem 8.3a
Describe three ways scientists use synthetic nucleic acids.
Problem 8.5a
Indicate which of the following are true and which are false. Rewrite any false statements to make them true by changing the underlined words.
__________ Gel electrophoresis is used in DNA microarrays.
Problem 8.5a
List three potential problems of recombinant DNA technology.
Problem 8.9a
Which application of recombinant DNA technology involves replacing a nonfunctional, mutated gene with a functional gene?
a. gene therapy
b. functional genomics
c. genetic screening
d. protein synthesis
Problem 11.7a
How is Agrobacterium used in recombinant DNA technology?
