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Ch.26 Nucleic Acids and Protein Synthesis
Chapter 26, Problem 2646

If a double-stranded DNA molecule is 22% G, what is the percentage of A, T, and C? Explain.

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1
Understand that in a double-stranded DNA molecule, the amount of guanine (G) is equal to the amount of cytosine (C), and the amount of adenine (A) is equal to the amount of thymine (T). This is due to the base pairing rules: G pairs with C, and A pairs with T.
Given that the DNA molecule is 22% guanine (G), it must also be 22% cytosine (C) because G pairs with C.
Calculate the combined percentage of G and C in the DNA molecule: 22% (G) + 22% (C) = 44%.
Since the total percentage of all bases in the DNA molecule must equal 100%, calculate the remaining percentage for adenine (A) and thymine (T): 100% - 44% = 56%.
Divide the remaining percentage equally between adenine (A) and thymine (T) because A pairs with T, so each must be 28%. Therefore, the DNA molecule is 28% adenine (A) and 28% thymine (T).

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Base Pairing Rules

In DNA, the base pairing rules dictate that guanine (G) pairs with cytosine (C), and adenine (A) pairs with thymine (T). This means that the amount of G in a double-stranded DNA molecule will equal the amount of C, and the amount of A will equal the amount of T. Understanding these rules is essential for calculating the percentages of the other bases when one is known.
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Chargaff's Rules

Chargaff's rules state that in a double-stranded DNA molecule, the ratio of adenine to thymine and the ratio of guanine to cytosine are equal. This principle helps in determining the composition of DNA, as knowing the percentage of one base allows for the calculation of the others. For example, if G is 22%, then C must also be 22%, leading to further deductions about A and T.
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Percentage Composition of DNA Bases

The percentage composition of DNA bases refers to the relative amounts of adenine, thymine, guanine, and cytosine in a DNA molecule. In a double-stranded DNA, the total percentage of A and T combined must equal the total percentage of G and C combined. This concept is crucial for solving problems related to base composition, as it allows for the calculation of unknown percentages based on known values.
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