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Ch. 5 - Chromosome Mapping in Eukaryotes
Chapter 5, Problem 13

Two different female Drosophila were isolated, each heterozygous for the autosomally linked genes b (black body), d (dachs tarsus), and c (curved wings). These genes are in the order d–b–c, with b being closer to d than to c. Shown here is the genotypic arrangement for each female along with the various gametes formed by both: Identify which categories are noncrossovers (NCOs), single crossovers (SCOs), and double crossovers (DCOs) in each case. Then, indicate the relative frequency in which each will be produced.

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insert step 1: Understand the genetic arrangement and linkage. The genes are arranged in the order d–b–c, with b closer to d than to c. This means that the likelihood of crossing over between d and b is less than between b and c.
insert step 2: Identify the noncrossover (NCO) gametes. These are the gametes that maintain the original parental arrangement of alleles without any crossing over.
insert step 3: Identify the single crossover (SCO) gametes. These are the gametes that result from a single crossover event between either d and b or b and c.
insert step 4: Identify the double crossover (DCO) gametes. These are the gametes that result from two crossover events, one between d and b and another between b and c.
insert step 5: Determine the relative frequency of each type of gamete. NCOs are typically the most frequent, followed by SCOs, and DCOs are the least frequent due to the requirement of two crossover events.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Genetic Linkage

Genetic linkage refers to the tendency of genes located close to each other on a chromosome to be inherited together during meiosis. This phenomenon affects the assortment of alleles and can lead to nonrandom combinations of traits in offspring. Understanding linkage is crucial for predicting gamete formation and the inheritance patterns of linked genes.
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Chi Square and Linkage

Types of Crossovers

Crossovers occur during meiosis when homologous chromosomes exchange genetic material. Noncrossovers (NCOs) result in gametes that are identical to the parental types, while single crossovers (SCOs) produce gametes with a mix of parental alleles. Double crossovers (DCOs) involve two crossover events and can result in gametes that are less frequent due to the complexity of the events involved.
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Recombination Frequency

Recombination frequency is a measure of how often crossovers occur between two genes during meiosis, influencing the genetic map distance between them. It is calculated based on the proportion of recombinant gametes produced compared to the total number of gametes. This frequency helps in determining the relative positions of genes on a chromosome and understanding the likelihood of different crossover types.
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Related Practice
Textbook Question
In a series of two-point mapping crosses involving five genes located on chromosome II in Drosophila, the following recombinant (single-crossover) frequencies were observed: pr–adp 29% pr–vg 13 pr–c 21 pr–b 6 adp–b 35 adp–c 8 adp–vg. 16 vg–b. 19 vg–c 8 c–b. 27 Given that the adp gene is near the end of chromosome II (locus 83), construct a map of these genes.
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Textbook Question
In a series of two-point mapping crosses involving five genes located on chromosome II in Drosophila, the following recombinant (single-crossover) frequencies were observed: pr–adp 29% pr–vg 13 pr–c 21 pr–b 6 adp–b 35 adp–c 8 adp–vg. 16 vg–b. 19 vg–c 8 c–b. 27 In another set of experiments, a sixth gene, d, was tested against b and pr: d–b 17% d–pr 23% Predict the results of two-point mapping between d and c, d and vg, and d and adp.
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Textbook Question
Three gene pairs located on separate autosomes determine flower color and shape as well as plant height. The first pair exhibits incomplete dominance, where the color can be red, pink (the heterozygote), or white. The second pair leads to personate (dominant) or peloric (recessive) flower shape, while the third gene pair produces either the dominant tall trait or the recessive dwarf trait. Homozygous plants that are red, personate, and tall are crossed to those that are white, peloric, and dwarf. Determine the F₁ genotype(s) and phenotype(s). If the F₁ plants are interbred, what proportion of the offspring will exhibit the same phenotype as the F₁ plants?
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Textbook Question
As in Problem 12, flower color may be red, white, or pink, and flower shape may be personate or peloric. For the following crosses, determine the P₁ and F₁ genotypes:
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Textbook Question
In Drosophila, a cross was made between females—all expressing the three X-linked recessive traits scute bristles (sc), sable body (s), and vermilion eyes (v)—and wild-type males. In the F₁, all females were wild type, while all males expressed all three mutant traits. The cross was carried to the F₂ generation, and 1000 offspring were counted, with the results shown in the following table. Phenotype Offspring sc s v 314 + + + 280 + s v 150 sc + + 156 sc + v 46 + s + 30 sc s + 10 + + v 14 No determination of sex was made in the data. Using proper nomenclature, determine the genotypes of the P₁ and F₁ parents.
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Textbook Question
In Drosophila, a cross was made between females—all expressing the three X-linked recessive traits scute bristles (sc), sable body (s), and vermilion eyes (v)—and wild-type males. In the F₁, all females were wild type, while all males expressed all three mutant traits. The cross was carried to the F₂ generation, and 1000 offspring were counted, with the results shown in the following table. Phenotype Offspring sc s v 314 + + + 280 + s v 150 sc + + 156 sc + v 46 + s + 30 sc s + 10 + + v 14 No determination of sex was made in the data. Determine the sequence of the three genes and the map distances between them.
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