Whole-exome sequencing (WES) is helping physicians diagnose a genetic condition that has defied diagnosis by traditional means. The implication here is that exons in the nuclear genome are sequenced in the hopes that, by comparison with the genomes of nonaffected individuals, a diagnosis might be revealed.

What are the strengths and weaknesses of this approach?

Video transcript

Hi everybody. Let's take a look at this practice problem together. Blank is a process of whole sequencing of a novel genome with no existing reference sequence. So a process of sequencing a genome for the very first time. So as the correct answer, a phases sequencing be de novo sequencing. See singer sequencing or D shotgun sequencing, I'd like to start off with C singer sequencing. In singer sequencing recall that primers are made specifically to buy next to known areas of interest. Therefore, a reference sequence is used and C is not the correct answer. In option D shotgun sequencing, a reference sequence is necessary and that reference sequence is a known genome recall that in this type of sequencing D N A is broken up into many small fragments that are then sequenced. A program assembles those sequence fragments in correct order if we have the known genome to compare it to. Therefore, option D is also not the correct answer. Option. A phases sequencing is not a known sequencing technique. So the correct answer is B de Novo sequencing is a process of whole sequencing of a novel genome with no existing reference sequence. Alright, everyone I hope you found this helpful and I'll see you soon for the next practice problem.