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Ch. 20 - Recombinant DNA Technology
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All textbooksKlug 12th EditionCh. 20 - Recombinant DNA TechnologyProblem 18

Chapter 19, Problem 18

The National Institutes of Health created the Genetic Testing Registry (GTR) to increase transparency by publicly sharing information about the utility of their tests, research for the general public, patients, health-care workers, genetic counselors, insurance companies, and others. The Registry is intended to provide better information to patients, but companies involved in genetic testing are not required to participate. Should company participation be mandatory? Why or why not? Explain your answers.

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Hello, everyone and welcome to today's video. So which of the following ethical concerns must be addressed first before conducting any genetic testing. And remember that these are the first thing that a researcher needs to worry about before anything is conducted. Well, as it turns out before we worry about making possible mistakes that might endanger someone's life or before we worry about protecting the intellectual property of the research we performed or we, before we worry about healthcare advanced directives, we need to worry about obtaining prior and informed consent from the person that we are studying without consent. None of these are going to be meaning anything because we are not able to perform the study. So answer choice C is going to be the correct answer to our question. I really hope this video helped you and I hope to see you on the next one.
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The Age of Genetics was created by remarkable advances in the use of biotechnology to manipulate plant and animal genomes. Given that the world population reached 7.5 billion people in 2017 and is expected to reach 9.7 billion in 2050, some scientists have proposed that only the worldwide introduction of genetically modified (GM) foods will increase crop yields enough to meet future nutritional demands. Pest resistance, herbicide, cold, drought, and salinity tolerance, along with increased nutrition, are seen as positive attributes of GM foods. However, others caution that unintended harm to other organisms, reduced effectiveness to pesticides, gene transfer to nontarget species, allergenicity, and as yet unknown effects on human health are potential concerns regarding GM foods. If you were in a position to control the introduction of a GM primary food product (rice, for example), what criteria would you establish before allowing such introduction?
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Variable number tandem repeats (VNTRs) are repeating DNA sequences of about 15–100 bp in length, found both within and between genes. Why are they commonly used in forensics?
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Textbook Question

To estimate the number of cleavage sites in a particular piece of DNA with a known size, you can apply the formula N/4ⁿ where N is the number of base pairs in the target DNA and n is the number of bases in the recognition sequence of the restriction enzyme. If the recognition sequence for BamHI is GGATCC and the  phage DNA contains approximately 48,500 bp, how many cleavage sites would you expect?

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Textbook Question

In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92-26 °C, (b) 45-65 °C, and (c) 65-75 °C.

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Should the FDA regulate direct-to-consumer genetic tests, or should these tests be available as a 'buyer beware' product?

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We usually think of enzymes as being most active at around 37°C, yet in PCR the DNA polymerase is subjected to multiple exposures of relatively high temperatures and seems to function appropriately at 65–75°C. What is special about the DNA polymerase typically used in PCR?

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