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Ch. 20 - Recombinant DNA Technology

Chapter 19, Problem 17

The Age of Genetics was created by remarkable advances in the use of biotechnology to manipulate plant and animal genomes. Given that the world population reached 7.5 billion people in 2017 and is expected to reach 9.7 billion in 2050, some scientists have proposed that only the worldwide introduction of genetically modified (GM) foods will increase crop yields enough to meet future nutritional demands. Pest resistance, herbicide, cold, drought, and salinity tolerance, along with increased nutrition, are seen as positive attributes of GM foods. However, others caution that unintended harm to other organisms, reduced effectiveness to pesticides, gene transfer to nontarget species, allergenicity, and as yet unknown effects on human health are potential concerns regarding GM foods. If you were in a position to control the introduction of a GM primary food product (rice, for example), what criteria would you establish before allowing such introduction?

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Hello, everyone. Welcome back. Let's look at our next problem. According to the researchers, the first synthetic bacterial genome was produced at the J Craig Ventner Institute in 2010. The first ever synthetic life form was given the name Blink Well, we have a little clue here. The first part of our question tells us that the first synthetic bacterial genome. So that lets us know that the first synthetic life form was a bacteria or bacterium, I should say created synthetic, completely synthetically in the lab. Now, one thing to note is that this was created from naturally occurring genes and not from any unique human sort of invented genes. But the it was significant in that this bacterial life itself was synthesized by humans in a lab. So the name given to this life form was Choice B Cynthia open there. And even if we didn't uh know for sure, we could guess again, we're assuming it's a bacterium. So Glow Fish, Choice C and Choice D knockout mouse, um you would eliminate the fact that they're not bacteria and choice A is dolly. Um You may recall that Dolly was the first cloned sheep. So she was a sheep, not a bacteria. So she was not a synthetic life form, but she was significant in being the first cloned animal. So not our answer, but we'll eliminate choice. So eliminate choice a dolly. And again, uh let's just look at our other answer choices to see that. Obviously, they're incorrect as they are not bacteria, but just to go through what they were. So we have choice C glow fish and these are genetically engineered fluorescent fish. So not synthetic life naturally occurring fish, but in a gene introduced into them that causes them to have fluorescence that they don't naturally have. And then finally, choice D knockout mouse kind of the opposite of the Glow Fish. A knockout mouse is generated when an existing gene in the mouse is destroyed or altered, using the introduction of artificial DNA into the genome. So you design a synthetic piece of DNA that's targeted to a specific gene area and you design it so that it will incorporate in a way such that it alters or destroys the gene that's present this way, you can study the effect of the absence of that gene or the alteration of that gene on the mouse that's so created. So again, we have the first ever synthetic life form given the name choice B Cynthia. See you in the next video.
Related Practice
Textbook Question

You have recovered a cloned DNA segment from a vector and determine that the insert is 1300 bp in length. To characterize this cloned segment, you isolate the insert and decide to construct a restriction map. Using enzyme I and enzyme II, followed by gel electrophoresis, you determine the number and size of the fragments produced by enzymes I and II alone and in combination, as recorded in the following table. Construct a restriction map from these data, showing the positions of the restriction-enzyme cutting sites relative to one another and the distance between them in units of base pairs.

Enzyme   Restriction Fragment Sizes (bp)
     I           350, 950
    II           200, 1100
I and II      150, 200, 950

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Textbook Question

To create a cDNA library, cDNA can be inserted into vectors and cloned. In the analysis of cDNA clones, it is often difficult to find clones that are full length—that is, many clones are shorter than the mature mRNA molecules from which they are derived. Why is this so?

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Textbook Question

Although the capture and trading of great apes has been banned in 112 countries since 1973, it is estimated that about 1000 chimpanzees are removed annually from Africa and smuggled into Europe, the United States, and Japan. This illegal trade is often disguised by simulating births in captivity. Until recently, genetic identity tests to uncover these illegal activities were not used because of the lack of highly polymorphic markers (markers that vary from one individual to the next) and the difficulties of obtaining chimpanzee blood samples. A study was reported in which DNA samples were extracted from freshly plucked chimpanzee hair roots and used as templates for PCR. The primers used in these studies flank highly polymorphic sites in human DNA that result from variable numbers of tandem nucleotide repeats. Several offspring and their putative parents were tested to determine whether the offspring were 'legitimate' or the product of illegal trading. The data are shown in the following Southern blot.

Examine the data carefully and choose the best conclusion.

a. None of the offspring are legitimate.
b. Offspring B and C are not the products of these parents and were probably purchased on the illegal market. The data are consistent with offspring A being legitimate.
c. Offspring A and B are products of the parents shown, but C is not and was therefore probably purchased on the illegal market.
d. There are not enough data to draw any conclusions. Additional polymorphic sites should be examined.
e. No conclusion can be drawn because 'human' primers were used. <>

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Textbook Question
Variable number tandem repeats (VNTRs) are repeating DNA sequences of about 15–100 bp in length, found both within and between genes. Why are they commonly used in forensics?
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Textbook Question

To estimate the number of cleavage sites in a particular piece of DNA with a known size, you can apply the formula N/4ⁿ where N is the number of base pairs in the target DNA and n is the number of bases in the recognition sequence of the restriction enzyme. If the recognition sequence for BamHI is GGATCC and the  phage DNA contains approximately 48,500 bp, how many cleavage sites would you expect?

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Textbook Question

The National Institutes of Health created the Genetic Testing Registry (GTR) to increase transparency by publicly sharing information about the utility of their tests, research for the general public, patients, health-care workers, genetic counselors, insurance companies, and others. The Registry is intended to provide better information to patients, but companies involved in genetic testing are not required to participate. Should company participation be mandatory? Why or why not? Explain your answers.

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