Describe the experimental rationale that allowed the lac repressor to be isolated.

Video transcript

Hi everyone. Let's take a look at this practice problem together. What technique did walter Gilbert and Beno moller. He'll use to isolate the partially purified form of lac repressor recall that lac repressor is a protein and it binds to DNA and represses the transcription of the lack opteron. So our answer will be a technique that isolates this protein or it can be used to isolate this protein. So let's take a look at our options. We've got B. Gram staining. Now, gram staining is used to identify and characterize bacteria based off of their cell wall composition. It does not isolate proteins and so B. Is incorrect. Then we've got C. Aseptic transfer. This is the method used in labs to ensure sample integrity and this is preventing contaminants or unwanted microorganisms from entering sterile samples and it does not isolate molecules so C. Is also incorrect. Then we've got Southern blotting as option D. Recall that blogging is a technique used to identify and quantify new click assets. Southern blotting specifically identifies D. N. A. But it does not isolate the D. N. A. Or the proteins and so it is also incorrect. So the correct answer is going to be a equilibrium dialysis. Now the definition of dialysis is the separation of particles and this is done using a membrane and so Gilbert and moller Hill were able to isolate that lac repressor. Using a dialysis back. So again. The answer is a equilibrium dialysis. Alright everyone I hope you found this helpful And I'll see you soon for the next practice problem