Predict what would happen to regulation of the lac operon if the lacI gene were moved 50,000 nucleotides upstream of its normal location.

Video transcript

Welcome back. Here's our next problem. Which of the following factors will not affect the regulation of black op aran. Well, let's focus on the fact that we're looking for what will not affect the regulation of this operation. So keep track of that as we work through our answer choices. The first one choice A says absence of activator or repressor. Well, this cannot be our answer because it will definitely effect the regulation. Because this is the very mechanism by which the expression of the genes is regulated in the absence of lactose. The repressor protein sits on the operator. It blocks the promoter and prevents the expression of those black jeans. When the repressor protein is removed, the activator protein, which is activated in the absence of glucose, helps promote the transcription of the lack genes in large amounts. So the presence or absence of activators and repressors are the ways in which this opera is regulated. So it definitely affects its regulation. And it's not our answer choice B says distance of the lac L jeans from the regulatory elements with black jeans or the jeans for the repressor protein. And this does not affect the regulation of the opera. And it doesn't matter how far the gene for the repressor protein is the regulator elements. Because that gene is expressed, it generates the repressor protein and that repressor protein is diffused through the cytoplasm of the cell. So it doesn't matter how far away. Um it's binding site is it's floating around in the cytoplasm and can still bind to its site. So how far the gene for the repressor protein is does not matter and will not affect the regulation of the opera choice C says absence of RNA polymerase. Well, this definitely will affect the regulation if there's no RNA polymerase, the DNA cannot be transcribed into RNA and those proteins cannot be be made eventually. So, absence of RNA polymerase definitely will affect regulation and not our answer. And finally, Choice D. The leader of deletion of the promoter region. The promoter region is where are in a preliminary spines to generate that M. R. N. A. So with the promoter region, isn't there? You're not going to express those genes. So, Choice D. Will definitely affect the regulation of the opera. So again, which the following factors will not affect the regulation of the lac opera choice be distance of the laC L jeans from the regulatory elements. See you in the next video