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Table of contents
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1. Introduction to Biochemistry
4h 34m
Worksheet
What is Biochemistry?
5m
Characteristics of Life
12m
Abiogenesis
13m
Nucleic Acids
16m
Proteins
12m
Carbohydrates
8m
Lipids
10m
Taxonomy
10m
Cell Organelles
12m
Endosymbiotic Theory
11m
Central Dogma
22m
Functional Groups
15m
Chemical Bonds
13m
Organic Chemistry
31m
Entropy
17m
Second Law of Thermodynamics
11m
Equilibrium Constant
10m
Gibbs Free Energy
37m
2. Water
3h 23m
Worksheet
Properties of Water
18m
Osmosis
18m
Hydrophobic Effect
16m
Acids and Bases
8m
Autoionization of Water
16m
pH
11m
Acid Dissociation Constant
19m
Henderson Hasselbalch Equation
27m
Determining Predominate Species
10m
Titration
35m
Buffer Solution
20m
3. Amino Acids
8h 10m
Worksheet
Amino Acid Groups
8m
Amino Acid Three Letter Code
13m
Amino Acid One Letter Code
37m
Amino Acid Configuration
20m
Essential Amino Acids
14m
Nonpolar Amino Acids
21m
Aromatic Amino Acids
14m
Polar Amino Acids
16m
Charged Amino Acids
40m
How to Memorize Amino Acids
1h 7m
Zwitterion
33m
Non-Ionizable Vs. Ionizable R-Groups
11m
Isoelectric Point
10m
Isoelectric Point of Amino Acids with Ionizable R-Groups
51m
Titrations of Amino Acids with Non-Ionizable R-Groups
44m
Titrations of Amino Acids with Ionizable R-Groups
38m
Amino Acids and Henderson-Hasselbalch
44m
4. Protein Structure
10h 4m
Worksheet
Peptide Bond
18m
Primary Structure of Protein
31m
Altering Primary Protein Structure
15m
Drawing a Peptide
44m
Determining Net Charge of a Peptide
42m
Isoelectric Point of a Peptide
37m
Approximating Protein Mass
7m
Peptide Group
22m
Ramachandran Plot
26m
Atypical Ramachandran Plots
12m
Alpha Helix
15m
Alpha Helix Pitch and Rise
20m
Alpha Helix Hydrogen Bonding
24m
Alpha Helix Disruption
23m
Beta Strand
12m
Beta Sheet
12m
Antiparallel and Parallel Beta Sheets
39m
Beta Turns
26m
Tertiary Structure of Protein
16m
Protein Motifs and Domains
23m
Denaturation
14m
Anfinsen Experiment
20m
Protein Folding
34m
Chaperone Proteins
19m
Prions
4m
Quaternary Structure
15m
Simple Vs. Conjugated Proteins
10m
Fibrous and Globular Proteins
11m
5. Protein Techniques
14h 5m
Worksheet
Protein Purification
7m
Protein Extraction
5m
Differential Centrifugation
15m
Salting Out
18m
Dialysis
9m
Column Chromatography
11m
Ion-Exchange Chromatography
35m
Anion-Exchange Chromatography
38m
Size Exclusion Chromatography
28m
Affinity Chromatography
16m
Specific Activity
16m
HPLC
29m
Spectrophotometry
51m
Native Gel Electrophoresis
23m
SDS-PAGE
34m
SDS-PAGE Strategies
16m
Isoelectric Focusing
17m
2D-Electrophoresis
23m
Diagonal Electrophoresis
29m
Mass Spectrometry
12m
Mass Spectrum
47m
Tandem Mass Spectrometry
16m
Peptide Mass Fingerprinting
16m
Overview of Direct Protein Sequencing
30m
Amino Acid Hydrolysis
10m
FDNB
26m
Chemical Cleavage of Bonds
29m
Peptidases
1h 6m
Edman Degradation
30m
Edman Degradation Sequenator and Sequencing Data Analysis
4m
Edman Degradation Reaction Efficiency
20m
Ordering Cleaved Fragments
21m
Strategy for Ordering Cleaved Fragments
58m
Indirect Protein Sequencing Via Geneomic Analyses
24m
6. Enzymes and Enzyme Kinetics
13h 38m
Worksheet
Enzymes
24m
Enzyme-Substrate Complex
17m
Lock and Key Vs. Induced Fit Models
23m
Optimal Enzyme Conditions
9m
Activation Energy
24m
Types of Enzymes
41m
Cofactor
15m
Catalysis
19m
Electrostatic and Metal Ion Catalysis
11m
Covalent Catalysis
18m
Reaction Rate
10m
Enzyme Kinetics
24m
Rate Constants and Rate Law
35m
Reaction Orders
52m
Rate Constant Units
11m
Initial Velocity
31m
Vmax Enzyme
27m
Km Enzyme
42m
Steady-State Conditions
25m
Michaelis-Menten Assumptions
18m
Michaelis-Menten Equation
52m
Lineweaver-Burk Plot
43m
Michaelis-Menten vs. Lineweaver-Burk Plots
20m
Shifting Lineweaver-Burk Plots
37m
Calculating Vmax
40m
Calculating Km
31m
Kcat
46m
Specificity Constant
1h 1m
7. Enzyme Inhibition and Regulation
8h 42m
Worksheet
Enzyme Inhibition
13m
Irreversible Inhibition
12m
Reversible Inhibition
9m
Inhibition Constant
26m
Degree of Inhibition
15m
Apparent Km and Vmax
29m
Inhibition Effects on Reaction Rate
10m
Competitive Inhibition
52m
Uncompetitive Inhibition
33m
Mixed Inhibition
40m
Noncompetitive Inhibition
26m
Recap of Reversible Inhibition
37m
Allosteric Regulation
7m
Allosteric Kinetics
17m
Allosteric Enzyme Conformations
33m
Allosteric Effectors
18m
Concerted (MWC) Model
25m
Sequential (KNF) Model
20m
Negative Feedback
13m
Positive Feedback
15m
Post Translational Modification
14m
Ubiquitination
19m
Phosphorylation
16m
Zymogens
13m
8. Protein Function
9h 41m
Worksheet
Introduction to Protein-Ligand Interactions
15m
Protein-Ligand Equilibrium Constants
22m
Protein-Ligand Fractional Saturation
32m
Myoglobin vs. Hemoglobin
27m
Heme Prosthetic Group
31m
Hemoglobin Cooperativity
23m
Hill Equation
21m
Hill Plot
42m
Hemoglobin Binding in Tissues & Lungs
31m
Hemoglobin Carbonation & Protonation
19m
Bohr Effect
23m
BPG Regulation of Hemoglobin
24m
Fetal Hemoglobin
6m
Sickle Cell Anemia
24m
Chymotrypsin
18m
Chymotrypsin's Catalytic Mechanism
38m
Glycogen Phosphorylase
21m
Liver vs Muscle Glycogen Phosphorylase
21m
Antibody
35m
ELISA
15m
Motor Proteins
14m
Skeletal Muscle Anatomy
22m
Skeletal Muscle Contraction
45m
9. Carbohydrates
7h 49m
Worksheet
Carbohydrates
19m
Monosaccharides
15m
Stereochemistry of Monosaccharides
33m
Monosaccharide Configurations
32m
Cyclic Monosaccharides
20m
Hemiacetal vs. Hemiketal
19m
Anomer
14m
Mutarotation
13m
Pyranose Conformations
23m
Common Monosaccharides
33m
Derivatives of Monosaccharides
21m
Reducing Sugars
21m
Reducing Sugars Tests
19m
Glycosidic Bond
48m
Disaccharides
40m
Glycoconjugates
12m
Polysaccharide
7m
Cellulose
7m
Chitin
8m
Peptidoglycan
12m
Starch
13m
Glycogen
14m
Lectins
16m
10. Lipids
5h 49m
Worksheet
Lipids
15m
Fatty Acids
30m
Fatty Acid Nomenclature
11m
Omega-3 Fatty Acids
12m
Triacylglycerols
11m
Glycerophospholipids
24m
Sphingolipids
13m
Sphingophospholipids
8m
Sphingoglycolipids
12m
Sphingolipid Recap
22m
Waxes
5m
Eicosanoids
19m
Isoprenoids
9m
Steroids
14m
Steroid Hormones
11m
Lipid Vitamins
19m
Comprehensive Final Lipid Map
13m
Biological Membranes
16m
Physical Properties of Biological Membranes
18m
Types of Membrane Proteins
8m
Integral Membrane Proteins
16m
Peripheral Membrane Proteins
12m
Lipid-Linked Membrane Proteins
21m
11. Biological Membranes and Transport
6h 37m
Worksheet
Biological Membrane Transport
21m
Passive vs. Active Transport
18m
Passive Membrane Transport
21m
Facilitated Diffusion
8m
Erythrocyte Facilitated Transporter Models
30m
Membrane Transport of Ions
29m
Primary Active Membrane Transport
15m
Sodium-Potassium Ion Pump
20m
SERCA: Calcium Ion Pump
10m
ABC Transporters
12m
Secondary Active Membrane Transport
12m
Glucose Active Symporter Model
19m
Endocytosis & Exocytosis
18m
Neurotransmitter Release
23m
Summary of Membrane Transport
21m
Thermodynamics of Membrane Diffusion: Uncharged Molecule
51m
Thermodynamics of Membrane Diffusion: Charged Ion
1h 1m
12. Biosignaling
9h 45m
Worksheet
Introduction to Biosignaling
44m
G protein-Coupled Receptors
32m
Stimulatory Adenylate Cyclase GPCR Signaling
42m
cAMP & PKA
28m
Inhibitory Adenylate Cyclase GPCR Signaling
29m
Drugs & Toxins Affecting GPCR Signaling
20m
Recap of Adenylate Cyclase GPCR Signaling
5m
Phosphoinositide GPCR Signaling
58m
PSP Secondary Messengers & PKC
27m
Recap of Phosphoinositide Signaling
7m
Receptor Tyrosine Kinases
26m
Insulin
28m
Insulin Receptor
23m
Insulin Signaling on Glucose Metabolism
57m
Recap Of Insulin Signaling in Glucose Metabolism
6m
Insulin Signaling as a Growth Factor
1h 1m
Recap of Insulin Signaling As A Growth Factor
9m
Recap of Insulin Signaling
1m
Jak-Stat Signaling
25m
Lipid Hormone Signaling
15m
Summary of Biosignaling
13m
Signaling Defects & Cancer
20m
Review 1: Nucleic Acids, Lipids, & Membranes
2h 47m
Worksheet
Nucleic Acids 1
9m
Nucleic Acids 2
11m
Nucleic Acids 3
4m
Nucleic Acids 4
4m
DNA Sequencing 1
9m
DNA Sequencing 2
11m
Lipids 1
11m
Lipids 2
4m
Membrane Structure 1
10m
Membrane Structure 2
9m
Membrane Transport 1
8m
Membrane Transport 2
4m
Membrane Transport 3
6m
Practice - Nucleic Acids 1
11m
Practice - Nucleic Acids 2
3m
Practice - Nucleic Acids 3
9m
Lipids
11m
Practice - Membrane Structure 1
7m
Practice - Membrane Structure 2
5m
Practice - Membrane Transport 1
6m
Practice - Membrane Transport 2
6m
Review 2: Biosignaling, Glycolysis, Gluconeogenesis, & PP-Pathway
3h 12m
Worksheet
Biosignaling 1
9m
Biosignaling 2
19m
Biosignaling 3
11m
Biosignaling 4
9m
Glycolysis 1
7m
Glycolysis 2
7m
Glycolysis 3
8m
Glycolysis 4
10m
Fermentation
6m
Gluconeogenesis 1
8m
Gluconeogenesis 2
7m
Pentose Phosphate Pathway
15m
Practice - Biosignaling
13m
Practice - Bioenergetics 1
10m
Practice - Bioenergetics 2
16m
Practice - Glycolysis 1
11m
Practice - Glycolysis 2
7m
Practice - Gluconeogenesis
5m
Practice - Pentose Phosphate Path
6m
Review 3: Pyruvate & Fatty Acid Oxidation, Citric Acid Cycle, & Glycogen Metabolism
2h 26m
Worksheet
Pyruvate Oxidation
9m
Citric Acid Cycle 1
14m
Citric Acid Cycle 2
7m
Citric Acid Cycle 3
7m
Citric Acid Cycle 4
11m
Metabolic Regulation 1
8m
Metabolic Regulation 2
13m
Glycogen Metabolism 1
6m
Glycogen Metabolism 2
8m
Fatty Acid Oxidation 1
11m
Fatty Acid Oxidation 2
8m
Citric Acid Cycle Practice 1
7m
Citric Acid Cycle Practice 2
6m
Citric Acid Cycle Practice 3
2m
Glucose and Glycogen Regulation Practice 1
4m
Glucose and Glycogen Regulation Practice 2
6m
Fatty Acid Oxidation Practice 1
4m
Fatty Acid Oxidation Practice 2
7m
Review 4: Amino Acid Oxidation, Oxidative Phosphorylation, & Photophosphorylation
1h 48m
Worksheet
Amino Acid Oxidation 1
5m
Amino Acid Oxidation 2
11m
Oxidative Phosphorylation 1
8m
Oxidative Phosphorylation 2
10m
Oxidative Phosphorylation 3
10m
Oxidative Phosphorylation 4
7m
Photophosphorylation 1
5m
Photophosphorylation 2
9m
Photophosphorylation 3
10m
Practice: Amino Acid Oxidation 1
2m
Practice: Amino Acid Oxidation 2
2m
Practice: Oxidative Phosphorylation 1
5m
Practice: Oxidative Phosphorylation 2
4m
Practice: Oxidative Phosphorylation 3
5m
Practice: Photophosphorylation 1
5m
Practice: Photophosphorylation 2
1m
8. Protein Function
Heme Prosthetic Group
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Heme Prosthetic Group
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