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Isoelectric Point

Isoelectric Point

29. Amino Acids / Isoelectric Point / Problem 1

Explain why glutamic acid (pI = 3.08) elutes before threonine (pI = 5.60) when their solution having pH 5 is passed through a cation-exchange column.

ANSWERS OPTIONS

A

Because glutamic acid has a higher amount of negative charge at this pH due to its lower pI value.

B

Because glutamic acid has a higher amount of positive charge at this pH due to its lower pI value.

C

Because threonine has a higher amount of negative charge at this pH due to its higher pI value.

D

Because threonine has a higher amount of positive charge at this pH due to its lower pI value.

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