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Isoelectric Point

Isoelectric Point

29. Amino Acids / Isoelectric Point / Problem 2

Explain why cysteine (pI = 5.15) elutes before isoleucine (pI = 5.60) when their solution having pH 5 is passed through a cation-exchange column.

ANSWERS OPTIONS

A

Because cysteine has a higher amount of positive charge at this pH due to its lower pI value

B

Because cysteine has a higher amount of negative charge at this pH due to its lower pI value

C

Because isoleucine has a higher amount of negative charge at this pH due to its higher pI value

D

Because isoleucine has a higher amount of positive charge at this pH due to its lower pI value

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