In 1996, scientists described a new tapeworm parasite that had killed at least one person. The initial examination of the patient’s abdominal mass was most likely made using
a. brightfield microscopy.
b. darkfield microscopy.
c. electron microscopy.
d. phase-contrast microscopy.
e. fluorescence microscopy.

Verified step by step guidance

1. Identify the nature of the sample being examined, which is an abdominal mass potentially containing a tapeworm parasite.
2. Consider the size and structure of the tapeworm parasite, which is a multicellular organism, and the need for detailed visualization.
3. Evaluate the capabilities of each microscopy technique: brightfield, darkfield, electron, phase-contrast, and fluorescence microscopy.
4. Brightfield microscopy is commonly used for observing stained or naturally pigmented specimens, providing a general view of the sample.
5. Electron microscopy offers high-resolution images and is suitable for detailed examination of small structures, but may not be necessary for initial examination of larger parasites.

Key Concepts

Microscopy Techniques

Microscopy techniques are essential tools in microbiology for visualizing microorganisms and cellular structures. Each technique has unique properties: brightfield microscopy uses light to illuminate samples, darkfield microscopy enhances contrast by scattering light, and phase-contrast microscopy allows for viewing live cells without staining. Understanding these methods helps in selecting the appropriate one for examining specific samples.

Brightfield Microscopy

Brightfield microscopy is the most common type of light microscopy, where light passes directly through the specimen, making it appear bright against a darker background. This method is effective for observing stained specimens or those with inherent color but may not provide sufficient contrast for transparent or colorless samples, such as live parasites.

Pathogen Identification

Pathogen identification is crucial in microbiology for diagnosing infections and understanding disease mechanisms. It often involves examining morphological characteristics, motility, and staining properties of the organism. In the case of a tapeworm, identifying its structure and reproductive features under a microscope can aid in confirming its presence and determining the appropriate treatment.