Suppose you want multiple copies of a gene you have synthesized. How would you obtain the necessary copies by cloning? By PCR?

You have a small gene that you want replicated by PCR. You add fluorescent dye-labeled nucleotides to the PCR thermal cycler. After three replication cycles, what percentage of the DNA single strands will fluoresce?

a. 0%

b. 12.5%

c. 50%

d. 87.5%

e. 100%

Match the following choices to the statements in questions 7 through 10.

a. antisense

b. clone

c. library

d. Southern blot

e. vector

Pieces of human DNA stored in yeast cells.

List at least two examples of the use of rDNA in medicine and in agriculture.

Match the following choices to the statements in questions 7 through 10.

a. antisense

b. clone

c. library

d. Southern blot

e. vector

A population of cells carrying a desired plasmid.

Match the following choices to the statements in questions 7 through 10.

a. antisense

b. clone

c. library

d. Southern blot

e. vector

Self-replicating DNA for transmitting a gene from one organism to another.

Which of the following organisms are most closely related? Are any two the same species? On what did you base your answer?

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Here is some additional information on the organisms in question 1:

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Which of these organisms are most closely related? Compare this answer with your response to review question 1.

Use the following choices to answer questions 9 and 10:

a. C. trachomatis

b. E. coli

c. Mycobacterium hominis

d. S. saprophyticus

In cases of NGU, diagnosis is made using PCR to detect microbial DNA.