DRAW IT Label components of the direct and indirect ELISA tests in the following situations. Which test is direct? Which test provides definitive proof of disease?

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Video transcript

Hey, everyone. Let's take a look at this question together. Why are enzyme linked antibodies used? Indirect el isa or drug detection? Is it answer choice A to increase the sensitivity of the test? Answer choice B to prevent drug binding to the well answer choice C to neutralize the drug in the urine sample or answer choice D to inhibit antibody antigen interactions. Let's work this problem out together to try to figure out which of the following answer choices best explains why enzyme linked antibodies are used in direct el isa for drug detection. So in order to solve this question, we have to recall what we have learned about direct el isa or drug detection to determine why enzyme linked antibodies would be used for drug detection. And we know that el ISA stands for enzyme linked immunosorbent assay. And we know that in enzyme linked immunosorbent assay, the enzyme linked antibodies bind directly to the target drug molecules present in the sample. And when a substrate specific to the enzyme is added, a detectable signal is produced allowing for the quantification of the drug concentration in the sample. And as a result, it amplifies the signal that is generated by the presence of the drug which then enhances the sensitivity of that test. So, enzyme linked antibodies are used in direct enzyme linked immunosorbent assay as it ultimately enhances the sensitivity of that test. So answer choice A is the correct answer and all other answer choices are incorrect. I hope you found this video to be helpful. Thank you and goodbye.