In an agglutination test, eight serial dilutions to determine antibody titer were set up: Tube 1 contained a 1:2 dilution; tube 2, a 1:4, and so on. If tube 5 is the last tube showing agglutination, what is the antibody titer?
a. 5
b. 1:5
c. 32
d. 1:32

Question

In an agglutination test, eight serial dilutions to determine antibody titer were set up: Tube 1 contained a 1:2 dilution; tube 2, a 1:4, and so on. If tube 5 is the last tube showing agglutination, what is the antibody titer?
a. 5
b. 1:5
c. 32
d. 1:32

a. 5

b. 1:5

c. 32

d. 1:32

Accepted Answer

Hey, everyone. Let's take a look at this question together out of six wells in micro tighter plates set for the direct agglutination test three showed agglutination. What will be the antibody tighter if the amount of serum that contains antibodies is diluted to a 1 to 20 dilution in the first? Well, is it answer choice A 1 to 80 choice B 80. Answer choice C 1 to 640 or answer choice D 640. Let's work this problem out together to try to figure out what the antibody tighter is if the amount of serum that contains antibodies is diluted to 1 to 20 in the first. Well, so in order to solve this question, we have to recall what we have learned about agglutination and how to find that antibody titer. To determine which of the following answer choices is the antibody titer. If the amount of serum that contains antibodies is diluted to 1 to 20 in the first. Well, out of six wells, and we can recall that the amount of particulate antigen in each well is the same in a direct agglutination test. However, the amount of s that contains antibodies is diluted. So that each successive, well has half of the antibodies of the previous. Well, so since we know that the first, well has 1 to 20 dilution. Well, one is that 1 to 20 dilution. And we know that each successive, well has half of the antibodies of the previous. Well, well, two would have a 1 to 40 dilution. Well, three would have a 1 to 80 dilution and then, well, four would be 1 to 160 then, well, five would be 1 to 320 so on where that number of antibodies is half of that previous. Well, and since we want to know the antibody tighter, if the amount of serum that contains antibodies is diluted to that 1 to 20 in the first. Well, we know that answer choice B 80 is the correct answer as 80 is the antibody tighter in this test as we know that the, well, with a 1 to 80 dilution is the most dilute concentration that still produces a positive reaction. So 80 would be the antibody tighter. So answer choice B is the only correct answer. Choice. I hope you found this video to be helpful. Thank you and goodbye.

In an agglutination test, eight serial dilutions to determine antibody titer were set up: Tube 1 contained a 1:2 dilution; tube 2, a 1:4, and so on. If tube 5 is the last tube showing agglutination, what is the antibody titer?
a. 5
b. 1:5
c. 32
d. 1:32

Verified Solution

Key Concepts

Agglutination Test

Serial Dilution

Antibody Titer

In an agglutination test, eight serial dilutions to determine antibody titer were set up: Tube 1 contained a 1:2 dilution; tube 2, a 1:4, and so on. If tube 5 is the last tube showing agglutination, what is the antibody titer?a. 5b. 1:5c. 32d. 1:32

Verified Solution

Key Concepts

Agglutination Test

Serial Dilution

Antibody Titer

In an agglutination test, eight serial dilutions to determine antibody titer were set up: Tube 1 contained a 1:2 dilution; tube 2, a 1:4, and so on. If tube 5 is the last tube showing agglutination, what is the antibody titer?
a. 5
b. 1:5
c. 32
d. 1:32