Textbook Question
Which one of the following temperatures would most likely kill a mesophile?
a. -50℃
b. 0℃
c. 9℃
d. 37℃
e. 60℃
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Ch. 6 - Microbial Growth
Chapter 6, Problem 6.9a
Which of the following types of media would not be used to culture aerobes?
a. selective media
b. reducing media
c. enrichment media
d. differential media
e. complex media
Verified step by step guidance1
Understand the characteristics of aerobes: Aerobes require oxygen to grow, so the media used should support oxygen presence.
Review the purpose of each type of media: Selective media allows the growth of certain microbes while inhibiting others; reducing media is used to grow anaerobes by reducing oxygen levels; enrichment media enhances the growth of specific microbes; differential media distinguishes between different microbes; complex media provides a variety of nutrients.
Identify which type of media is specifically designed to reduce oxygen levels, as this would not support the growth of aerobes.
Recognize that reducing media is used to culture anaerobes, which do not require oxygen, and thus would not be suitable for aerobes.
Conclude that reducing media is the type of media that would not be used to culture aerobes.
Verified Solution
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Aerobic Microorganisms
Aerobic microorganisms require oxygen for their growth and metabolism. They utilize oxygen as the final electron acceptor in cellular respiration, which allows them to generate energy efficiently. Understanding the requirements of aerobes is crucial for selecting appropriate culture media that support their growth.
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01:25
Importance of Microorganisms
Types of Culture Media
Culture media can be classified into several types, including selective, differential, enrichment, and reducing media. Selective media favor the growth of specific organisms while inhibiting others, differential media allow for the differentiation of organisms based on biochemical characteristics, and enrichment media enhance the growth of particular organisms. Reducing media, on the other hand, are designed to create an oxygen-free environment, which is unsuitable for aerobes.
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02:42Introduction to the Types of Culture Media
Reducing Media
Reducing media are specifically formulated to remove oxygen, creating an anaerobic environment. They contain reducing agents that chemically bind oxygen, making them ideal for culturing anaerobic bacteria. Since aerobes thrive in the presence of oxygen, reducing media would not be appropriate for their culture, highlighting the importance of understanding the specific needs of different microorganisms.
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02:55Reducing Media
Related Practice
Textbook Question
Differentiate complex and chemically defined media.
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Textbook Question
Which of the following is true about the optimum growth temperature of most mesophiles?
a) They can grow well at 37^0C.
b) They are more likely to grow at refrigerator temperatures.
c) They can strive well in extremely high temperatures.
d) All of the above.
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Textbook Question
The thermal death time for a suspension of Bacillus subtilis endospores is in dry heat and less than in an autoclave. Which type of heat is more effective? Why?
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Textbook Question
If pasteurization does not achieve sterilization, why is pasteurization used to treat food?
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Textbook Question
Thermal death point is not considered an accurate measure of the effectiveness of heat sterilization. List three factors that can alter thermal death point.
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