Acetobacter is necessary for only one of the steps of vitamin C manufacture. The easiest way to accomplish this step would be to
a. add substrate and Acetobacter to a test tube.
b. affix Acetobacter to a surface and run substrate over it.
c. add substrate and Acetobacter to a bioreactor.
d. find an alternative to this step.
e. none of the above

Question

Acetobacter is necessary for only one of the steps of vitamin C manufacture. The easiest way to accomplish this step would be to

a. add substrate and Acetobacter to a test tube.

b. affix Acetobacter to a surface and run substrate over it.

c. add substrate and Acetobacter to a bioreactor.

d. find an alternative to this step.

e. none of the above

Accepted Answer

Hi, everybody and welcome back. Here's our next question. Suppose you want to produce a large quantity of penicillin through an industrial fermentation process, which of the following steps is not essential in achieving your goal. A adding correct growth medium, B adding necessary enzymes for penicillin production. C inoculating the bio reactor with an inoculum from the penicillium chrysogenum culture D setting the bioreactor to appropriate parameters or E none of the above. So let's think about this process and what you're going to be doing, you're going to be growing a large culture of the fungus that produces penicillin, which is penicillium chrysogenum. And so you'll want optimal conditions for maximizing your yield when you're making something on an industrial scale. A small difference in what percent yield you get can make a big difference in your profitability in the amount you're producing. So you want to really optimize these conditions. So we want to find out which of these steps are not essential to make this large quantity of penicillin, but we do have the option that all of them are essential. None of the above. In this case would mean they're all essential. So let's just evaluate each of our steps to say, adding the correct growth medium. Well, that's pretty essential. You need to have the right amount of nutrients to allow maximal growth of your penicillium priso. If your fungus runs out of one or another essential nutrient, it won't be able to produce any more penicillin. So choice A is not our answer because that will be essential than choice. B says adding the necessary enzymes for penicillin production. Well, certain enzymes are needed to produce penicillin, but you don't need to add them to your culture. These, you're going to be using the penicillin, priso cells as little tiny factories because they make their own penicillin as part of their normal metabolic process. They already have these enzymes to make it. So there's no need to add them to the culture. So step B would be our step. That is not necessary. We can then go ahead and eliminate choice. E since we've found a not an unnecessary step just to be thorough. Let's look at our other answer. Choices. Choice C inoculating the bio reactor with an inoculum for the penicillium chrysogenum culture. Well, you've got to put the fungus in there in the first place. If you don't have these cells, you can't make penicillin. So choice C definitely necessary. And then choice D setting the bio reactor to appropriate para parameters just as you needed the right kind of nutrients to maximize your yield. You'll want the right conditions for them to have the best growth. So that would include things like having the optimal temperature ph amount of dissolved oxygen, the agitation rate and of course the nutrient supply. So choice d definitely necessary and not our answer. So once again, the answer choice here, that's not essential to achieve your goal of producing a large quantity of penicillin through an industrial fermentation process is choice B adding necessary enzymes for penicillin production. See you in the next video.

Verified Solution

Key Concepts

Role of Acetobacter in Vitamin C Production

Bioreactor Functionality

Substrate Utilization