Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages
All textbooksKlug 12th EditionCh. 6 - Genetic Analysis and Mapping in Bacteria and BacteriophagesProblem 22
Chapter 6, Problem 22
In studies of recombination between mutants 1 and 2 from Problem 21, the results shown in the following table were obtained. Strain Dilution Plaques Phenotypes E. coli B 10⁻⁷ 4 r E. coli K12 10⁻² 8 + Mutant 7 (Problem 21) failed to complement any of the other mutants (1–6). Define the nature of mutant 7.
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Textbook Question
During the analysis of seven rII mutations in phage T4, mutants 1, 2, and 6 were in cistron A, while mutants 3, 4, and 5 were in cistron B. Of these, mutant 4 was a deletion overlapping mutant 5. The remainder were point mutations. Nothing was known about mutant 7. Predict the results of complementation (+ or -) between 1 and 2; 1 and 3; 2 and 4; and 4 and 5.
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Textbook Question
In studies of recombination between mutants 1 and 2 from Problem 21, the results shown in the following table were obtained.
Strain Dilution Plaques Phenotypes
E. coli B 10⁻⁷ 4 r
E. coli K12 10⁻² 8 +
Calculate the recombination frequency.
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Textbook Question
In studies of recombination between mutants 1 and 2 from Problem 21, the results shown in the following table were obtained.
Strain Dilution Plaques Phenotypes
E. coli B 10⁻⁷ 4 r
E. coli K12 10⁻² 8 +
When mutant 6 was tested for recombination with mutant 1, the data were the same as those shown above for strain B, but not for K12. The researcher lost the K12 data, but remembered that recombination was ten times more frequent than when mutants 1 and 2 were tested. What were the lost values (dilution and plaque numbers)?
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Textbook Question
In Bacillus subtilis, linkage analysis of two mutant genes affecting the synthesis of two amino acids, tryptophan (trp₂⁻), and tyrosine (trp₁⁻), was performed using transformation. Examine the following data and draw all possible conclusions regarding linkage. What is the purpose of Part B of the experiment? [Reference: E. Nester, M. Schafer, and J. Lederberg (1963).]
Donor DNA Recipient Cell Transformants No.
trp⁺ tyr⁻ 196
A. trp₂⁺ tyr₁⁺ trp₂⁻ tyr₁⁻ trp⁻ tyr⁺ 328
trp⁺ tyr⁺ 367
trp₂⁺ tyr₁⁻ trp⁺ tyr⁻ 190
B. and trp₂⁻ tyr₁⁻ trp⁻ tyr⁺ 256
trp₂⁻ tyr₁⁺ trp⁺ tyr⁺ 2
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Textbook Question
An Hfr strain is used to map three genes in an interrupted mating experiment. The cross is Hfr/a⁺b⁺c⁺ rif x F⁻/a⁻b⁻c⁻ rif^T (No map order is implied in the listing of the alleles; rif^T is resistance to the antibiotic rifampicin.) The a⁺ gene is required for the biosynthesis of nutrient A, the b⁺ gene for nutrient B, and c⁺ for nutrient C. The minus alleles are auxotrophs for these nutrients. The cross is initiated at time = 0 and at various times, the mating mixture is plated on three types of medium. Each plate contains minimal medium (MM) plus rifampicin plus specific supplements that are indicated in the following table. (The results for each time interval are shown as the number of colonies growing on each plate.)
Time of Interruption _
5 min 10 min 15 min 20 min
Nutrients A and B 0 0 4 21
Nutrients B and C 0 5 23 40
Nutrients A and C 4 25 60 82
What is the purpose of rifampicin in the experiment?
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Textbook Question
An Hfr strain is used to map three genes in an interrupted mating experiment. The cross is Hfr/a⁺b⁺c⁺ rif x F⁻/a⁻b⁻c⁻ rif^T (No map order is implied in the listing of the alleles; rif^T is resistance to the antibiotic rifampicin.) The a⁺ gene is required for the biosynthesis of nutrient A, the b⁺ gene for nutrient B, and c⁺ for nutrient C. The minus alleles are auxotrophs for these nutrients. The cross is initiated at time = 0 and at various times, the mating mixture is plated on three types of medium. Each plate contains minimal medium (MM) plus rifampicin plus specific supplements that are indicated in the following table. (The results for each time interval are shown as the number of colonies growing on each plate.)
Time of Interruption _
5 min 10 min 15 min 20 min
Nutrients A and B 0 0 4 21
Nutrients B and C 0 5 23 40
Nutrients A and C 4 25 60 82
Based on these data, determine the approximate location on the chromosome of the a, b, and c genes relative to one another and to the F factor.
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