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Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages

Chapter 6, Problem 17

In recombination studies of the rII locus in phage T4, what is the significance of the value determined by calculating phage growth in the K12 versus the B strains of E. coli following simultaneous infection in E. coli B? Which value is always greater?

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Hello everyone and welcome to today's video. So the transfer of genetic material from parent to offspring is referred to as blank. However, the transfer of genetic material from one bacterium to another is called as blank. Well, in order to solve this problem, we just need to visualize what these transfers are going to look like. Let's say that we have apparent organism and then we have the the offspring of this organism. But we also have an organism that's in the same generation as the parents. Now, if we're transferring as the first sentence says genetic material from pairing to offspring, we can see that this is going to be transferred vertically because of this. This is termed as vertical gene transfer. However, when we're transferring genetic material from the parent to someone else in its in its same generation, then we're transferring or horizontally meaning that this is going to be called horizontal gene transfer. And this is going to be represented by answer choice. So the transfer of genetic material from praying to offspring is referred to as vertical gene transfer. However, the transfer of genetic material from one bacterium to another is called horizontal gene transfer. I really hope this video helped you and I hope to see you on the next one
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Textbook Question
The bacteriophage genome consists of many genes encoding proteins that make up the head, collar, tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?
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Textbook Question
If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?
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Textbook Question
If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur? Dilution Factor Assay Results (a) 10⁴ All bacteria lysed (b) 10⁵ 14 plaques (c) 10⁶ 0 plaques
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Textbook Question
In an analysis of rII mutants, complementation testing yielded the following results: Mutants Results (+/- lysis) 1, 2 + 1, 3 + 1, 4 - 1, 5 - Predict the results of testing 2 and 3, 2 and 4, and 3 and 4 together.
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Textbook Question
If further testing of the mutations in Problem 18 yielded the following results, what would you conclude about mutant 5? Mutants Results 2, 5 - 3, 5 - 4, 5 -
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Textbook Question
Using mutants 2 and 3 from Problem 19, following mixed infection on E. coli B, progeny viruses were plated in a series of dilutions on both E. coli B and K12 with the following results. What is the recombination frequency between the two mutants? Strain Plated Dilution Plaques E. coli B 10⁻⁵ 2 E. coli K12 10⁻¹ 5
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