Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages
All textbooksKlug 12th EditionCh. 6 - Genetic Analysis and Mapping in Bacteria and BacteriophagesProblem 17
Chapter 6, Problem 17
In recombination studies of the rII locus in phage T4, what is the significance of the value determined by calculating phage growth in the K12 versus the B strains of E. coli following simultaneous infection in E. coli B? Which value is always greater?
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Textbook Question
The bacteriophage genome consists of many genes encoding proteins that make up the head, collar, tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?
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Textbook Question
If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?
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Textbook Question
If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?
Dilution Factor Assay Results
(a) 10⁴ All bacteria lysed
(b) 10⁵ 14 plaques
(c) 10⁶ 0 plaques
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Textbook Question
In an analysis of rII mutants, complementation testing yielded the following results:
Mutants Results (+/- lysis)
1, 2 +
1, 3 +
1, 4 -
1, 5 -
Predict the results of testing 2 and 3, 2 and 4, and 3 and 4 together.
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Textbook Question
If further testing of the mutations in Problem 18 yielded the following results, what would you conclude about mutant 5?
Mutants Results
2, 5 -
3, 5 -
4, 5 -
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Textbook Question
Using mutants 2 and 3 from Problem 19, following mixed infection on E. coli B, progeny viruses were plated in a series of dilutions on both E. coli B and K12 with the following results.
What is the recombination frequency between the two mutants?
Strain Plated Dilution Plaques
E. coli B 10⁻⁵ 2
E. coli K12 10⁻¹ 5
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