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Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages

Chapter 6, Problem 18

In an analysis of rII mutants, complementation testing yielded the following results: Mutants Results (+/- lysis) 1, 2 + 1, 3 + 1, 4 - 1, 5 - Predict the results of testing 2 and 3, 2 and 4, and 3 and 4 together.

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Hello, everyone. Here we have a question asking what is the difference between the R two Mutant pages and the Wild type pages? So the difference between the R two Mutant pages and the Wild type pages is whether or not they can Leis E Coli B or E Coli K 12. So the R two Mutant and the Wild type can both wise E coli B. So both E Coli B but only the wild type can lies the E Coli K Because the R two mutant cannot. So our answer here is D The mutant type can only lie sequelae B but could not lice equal IK 12. Thank you for watching. Bye.
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If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?
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Textbook Question
If a single bacteriophage infects one E. coli cell present on a lawn of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur? Dilution Factor Assay Results (a) 10⁴ All bacteria lysed (b) 10⁵ 14 plaques (c) 10⁶ 0 plaques
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In recombination studies of the rII locus in phage T4, what is the significance of the value determined by calculating phage growth in the K12 versus the B strains of E. coli following simultaneous infection in E. coli B? Which value is always greater?
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If further testing of the mutations in Problem 18 yielded the following results, what would you conclude about mutant 5? Mutants Results 2, 5 - 3, 5 - 4, 5 -
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Using mutants 2 and 3 from Problem 19, following mixed infection on E. coli B, progeny viruses were plated in a series of dilutions on both E. coli B and K12 with the following results. What is the recombination frequency between the two mutants? Strain Plated Dilution Plaques E. coli B 10⁻⁵ 2 E. coli K12 10⁻¹ 5
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Textbook Question
Using mutants 2 and 3 from Problem 19, following mixed infection on E. coli B, progeny viruses were plated in a series of dilutions on both E. coli B and K12 with the following results. Another mutation, 6, was tested in relation to mutations 1 through 5 from Problems 18–20. In initial testing, mutant 6 complemented mutants 2 and 3. In recombination testing with 1, 4, and 5, mutant 6 yielded recombinants with 1 and 5, but not with 4. What can you conclude about mutation 6?
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