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Ch. 20 - Recombinant DNA Technology

Chapter 19, Problem 13

Describe how the team from the J. Craig Venter Institute created a synthetic genome. How did the team demonstrate that the genome converted the recipient strain of bacteria into a different strain?

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Hello, everyone and welcome to today's video. So why should the creation of synthetic genomes be regulated? Well, before we jump into our answer, choices, I really want you to think about this in the following way. What if we were replicating or creating a synthetic genome of the Polio virus? And we made a mistake and without realizing we are creating something worse. We're creating some type of biological weapon. This is going to affect millions of people and could be very destructive towards humanity because of this synthetic genomes need to be regulated. Now, let's look over each of our answer traces and see which one is going to align with this province. But we have to preserve their primitive genetic makeup. This is not something that is going to be affected by creating synthetic genomes as they use the same genetic makeup as primitive genomes. So we're going to cancel this out. Then we have c to control unnecessary species evolution. We're creating a synthetic genome will be like creating just another member of the species. This is not going to lead to species evolution. So we're going to cancel this artist. Well, then we have to maintain the regional trade over time. Again, this is not going to lead to evolution. So we're going to cancel this out and then we have a due to the significant threat in case of misapplication. And this is going to apply to the Polio virus example that I posed for you earlier. If we create, if we're trying to create this synthetic genes of the Polio virus, and we made a mistake, we could create a bio weapon that could be very destructive towards humanity. So answer choice. A is a correct answer as to why synthetic genomes need to be regulated. I really hope this helped you and I hope to see you on the next one.
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What advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.

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You have recovered a cloned DNA segment from a vector and determine that the insert is 1300 bp in length. To characterize this cloned segment, you isolate the insert and decide to construct a restriction map. Using enzyme I and enzyme II, followed by gel electrophoresis, you determine the number and size of the fragments produced by enzymes I and II alone and in combination, as recorded in the following table. Construct a restriction map from these data, showing the positions of the restriction-enzyme cutting sites relative to one another and the distance between them in units of base pairs.

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