Textbook Question
Explain how the following mutations would affect transcription of the yeast GAL1 gene in the presence of galactose.
A point mutation in the GAL1 core promoter that alters the sequence of the TATA box.
204
views
Ch. 17+18 - Transcriptional Regulation in Eukaryotes
Chapter 17, Problem 22
What role do ubiquitin ligases play in the regulation of gene expression?
Verified step by step guidance1
Ubiquitin ligases are enzymes that attach ubiquitin molecules to proteins, marking them for degradation by the proteasome.
This process is known as ubiquitination, and it is crucial for regulating the levels of various proteins within the cell.
By controlling the degradation of transcription factors and other regulatory proteins, ubiquitin ligases can influence gene expression.
For example, if a transcription factor is ubiquitinated and degraded, the genes it regulates may be expressed at lower levels.
Conversely, preventing the degradation of a transcription factor can lead to increased expression of its target genes.
Verified Solution
Video duration:
2m
This video solution was recommended by our tutors as helpful for the problem above.Was this helpful?
Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Ubiquitin-Proteasome System
The ubiquitin-proteasome system is a cellular mechanism that tags proteins for degradation. Ubiquitin, a small protein, is attached to target proteins by ubiquitin ligases, marking them for destruction by the proteasome. This process is crucial for regulating protein levels and maintaining cellular homeostasis, impacting various cellular functions, including gene expression.
Recommended video:
Guided course
04:30
Post Translational Modifications
Ubiquitin Ligases
Ubiquitin ligases are enzymes that facilitate the attachment of ubiquitin to specific substrate proteins. They play a critical role in determining which proteins are marked for degradation, thus influencing the availability of transcription factors and other regulatory proteins that can affect gene expression. Their activity can be modulated by various signals, linking cellular responses to gene regulation.
Recommended video:
Guided course
04:30Post Translational Modifications
Gene Expression Regulation
Gene expression regulation refers to the processes that control the timing and amount of gene product (RNA or protein) produced in a cell. This regulation can occur at multiple levels, including transcription, RNA processing, and translation. Ubiquitin ligases contribute to this regulation by controlling the stability and activity of proteins involved in transcription, thereby influencing the overall expression of genes.
Recommended video:
Guided course
02:09Penetrance and Expressivity
Related Practice
Textbook Question
Explain how the following mutations would affect transcription of the yeast GAL1 gene in the presence of galactose.
A mutation within the GAL80 gene that blocks the ability of Gal80 protein to interact with Gal3p.
334
views
Textbook Question
Explain how the following mutations would affect transcription of the yeast GAL1 gene in the presence of galactose.
A deletion of one of the four UASG elements upstream from the GAL1 gene.
272
views
Textbook Question
Explain how the following mutations would affect transcription of the yeast GAL1 gene in the presence of galactose.
A deletion within the GAL4 gene that removes the region encoding amino acids 1 to 100.
231
views
Textbook Question
Explain how the following mutations would affect transcription of the yeast GAL1 gene in the presence of galactose.
A deletion of the entire GAL3 gene.
173
views
Textbook Question
Much of what we know about gene interactions in development has been learned using nematodes, yeast, flies, and bacteria. This is due, in part, to the relative ease of genetic manipulation of these well-characterized genomes. However, of great interest are gene interactions involving complex diseases in humans. Wang and White [(2011). Nature Methods 8(4):341–346] describe work using RNAi to examine the interactive proteome in mammalian cells. They mention that knockdown inefficiencies and off-target effects of introduced RNAi species are areas that need particular improvement if the methodology is to be fruitful.
How might one use RNAi to study developmental pathways?
257
views