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Ch. 11 - DNA Replication and Recombination

Chapter 11, Problem 18

Why is DNA synthesis expected to be more complex in eukaryotes than in bacteria? How is DNA synthesis similar in the two types of organisms?

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Everyone. Let's take a look at this question together, which of the following characteristics of DNA replication best describes the similarities between pro carry outs and you carry outs. So let's recall how DNA replication is done in both pro carry outs and you carry outs to compare them to figure out which of the following characteristics Best describes the similarities between them. So let's start off by talking about the pro carry out And how DNA replication is done in precarious when we know that it is done in a 5- direction with the nuclear tides added 23, and we also know that it is I directional and lastly we know that replication is done before cell division and this is to ensure that there is successful genetic inheritance. And so, talking about you carry out on the other hand, we know that it is also done in that 5 to 3 direction with the nuclear tides added to the 30. H. And that it is also by directional as well as that replication before the vision for the same reason as the pro carry is to ensure that successful genetic inheritance. So we can see that DNA replication between pro carry oats and eukaryotes is very similar and looking at our answer choices, answer choices A B and C are all characteristics of DNA replication that are similar between those pro and eukaryotes. So our best answer to this question is answer choice D all the above because we know that replication occurs in that 5 to 3 direction, that it is bi directional and that replication occurs before that division. So answer choice D. Is the correct answer. I hope you found this video to be helpful. Thank you and goodbye.
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Outline the current model for DNA synthesis.
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Suppose that E. coli synthesizes DNA at a rate of 100,000 nucleotides per minute and takes 40 minutes to replicate its chromosome. (a) How many base pairs are present in the entire E. coli chromosome? (b) What is the physical length of the chromosome in its helical configuration—that is, what is the circumference of the chromosome if it were opened into a circle?
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Textbook Question

DNA supercoiling, which occurs when coiling tension is generated ahead of the replication fork, is relieved by DNA gyrase. Supercoiling may also be involved in transcription regulation. Researchers discovered that enhancers operating over a long distance (2500 bp) are dependent on DNA supercoiling, while enhancers operating over shorter distances (110 bp) are not so dependent [Liu et al. (2001). Proc. Natl. Acad. Sci. USA 98:14,883–14,888]. Using a diagram, suggest a way in which supercoiling may positively influence enhancer activity over long distances.

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Several temperature-sensitive mutant strains of E. coli display the following characteristics. Predict what enzyme or function is being affected by each mutation. Newly synthesized DNA contains many mismatched base pairs.
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