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Ch. 20 - DNA Tools and Biotechnology
Chapter 20, Problem 3

Plants are more readily manipulated by genetic engineering than are animals because a. plant genes do not contain introns. b. more vectors are available for transferring recombinant DNA into plant cells. c. a somatic plant cell can often give rise to a complete plant. d. plant cells have larger nuclei.

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1
Identify the key terms in the question: genetic engineering, manipulated, plant cells, animal cells.
Understand the role of somatic cells in plants: Somatic plant cells have the ability to differentiate into a complete plant, a process known as totipotency.
Evaluate the options in relation to genetic engineering: Option c (a somatic plant cell can often give rise to a complete plant) directly relates to the ease of manipulating plants genetically because individual cells can be modified and then grown into a new plant, expressing the desired traits.
Compare with other options: Options a, b, and d do not directly influence the ease of manipulation as much as the totipotency of plant cells does.
Conclude that the correct answer is c because the ability of a somatic plant cell to give rise to a complete plant simplifies the process of genetic engineering in plants, making them more readily manipulated than animals.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Genetic Engineering in Plants

Genetic engineering involves modifying an organism's DNA to achieve desired traits. In plants, this process is often facilitated by techniques such as Agrobacterium-mediated transformation or biolistics, which allow for the introduction of new genes. The ability to regenerate whole plants from single somatic cells makes plants particularly amenable to genetic manipulation.
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Plant Cell Totipotency

Totipotency refers to the ability of a single cell to develop into a complete organism. In plants, somatic cells can often regenerate into whole plants, allowing for the incorporation of genetic modifications at the cellular level. This characteristic is a key reason why plants are more easily manipulated through genetic engineering compared to animals.
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Vectors in Genetic Engineering

Vectors are tools used to deliver genetic material into target cells. In plant genetic engineering, various vectors, such as plasmids and viral vectors, are employed to transfer recombinant DNA into plant cells. The availability of multiple effective vectors enhances the efficiency of genetic modifications in plants, making the process more straightforward than in animal cells.
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Related Practice
Textbook Question

In DNA technology, the term vector can refer to a. the enzyme that cuts DNA into restriction fragments. b. the sticky end of a DNA fragment. c. a SNP marker. d. a plasmid used to transfer DNA into a living cell.

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Textbook Question

Which of the following tools of DNA technology is incorrectly paired with its use? a. electrophoresis—separation of DNA fragments b. DNA ligase—cutting DNA, creating sticky ends of restriction fragments c. DNA polymerase—polymerase chain reaction to amplify sections of DNA d. reverse transcriptase—production of cDNA from mRNA

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Textbook Question

A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird). To compare a specific region of the DNA from a sample with DNA from living birds, which of the following would be most useful for increasing the amount of dodo DNA available for testing? a. SNP analysis b. polymerase chain reaction (PCR) c. electroporation d. gel electrophoresis

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Textbook Question
DNA technology has many medical applications. Which of the following is not done routinely at present? a. production of hormones for treating diabetes and dwarfism b. analysis of gene expression for more informed cancer treatments c. gene editing by the CRISPR-Cas9 system in viable human embryos to correct genetic diseases d. prenatal identification of genetic disease alleles
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Textbook Question

Which of the following is not true of cDNA produced using human brain tissue as the starting material? a. It can be amplified by the polymerase chain reaction. b. It was produced from pre-mRNA using reverse transcriptase. c. It can be labeled and used as a probe to detect genes expressed in the brain. d. It lacks the introns of the pre-mRNA.

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