Here are the essential concepts you must grasp in order to answer the question correctly.
Enzyme Saturation
Enzyme saturation occurs when all active sites of the enzyme molecules are occupied by substrate, leading to a maximum reaction rate. At this point, adding more substrate does not increase the rate of product formation, as the enzyme is already working at full capacity.
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Enzyme Concentration
Increasing the concentration of the enzyme can enhance the reaction rate when the substrate is saturated. More enzyme molecules mean more active sites available for substrate binding, allowing for a higher yield of products, provided that substrate availability is not a limiting factor.
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Noncompetitive Inhibition
Noncompetitive inhibitors bind to an enzyme regardless of whether the substrate is present, reducing the overall number of active enzyme molecules. This type of inhibition does not increase the reaction rate and can lead to a decrease in product yield, making it an ineffective choice when the enzyme is already saturated.
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