Match each of the following tests in column A to its positive reaction in column B.
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Question

Match each of the following tests in column A to its positive reaction in column B.

Accepted Answer

Hi and welcome back. Our next question says which serological test is utilized in the pregnancy test? A agglutination test. B Western blotting C Eliza or D precipitation test. Well, we can get to the idea of which test we'd use if we think about what you would want to test for, to test for pregnancy. And the answer is a specific protein called HCG or human chorionic gonadotropin, which is only produced by the body during pregnancy, not any other time. And of course, when you're looking for one specific protein, a very efficient way to do that is utilizing an antibody, which would of course be specific to that protein. So which of our tests involved the use of an antibody to identify proteins. And there's two of them which is choice b, Western blotting and choice C Eliza. So both involve using labeled antibodies or labeled antigens in the case of Eliza to identify whether or not something is present. However, western blotting involves running a gel and transferring it to paper and then washing that paper with labeled antibodies to detect where the specific protein is present. And so obviously, this is not a good procedure to do for a home pregnancy test. This is a laboratory procedure. So choice B would identify the presence of HTD HCG. But it's not practical for people to use at home to identify pregnancy. However, choice C Eliza and Eliza assay is very simple to use. It's often used in a lab, scaled up to large numbers of samples but can be used for a single sample at home. And in the case of a pregnancy test, you have antibodies to HCG that are immobilized on a solid surface like a plastic well or a stick urine is added. If the urine contains HCG, that HCG will bind to the antibody. You have then another labeled enzyme that's specific to HCG and some sort of color changing agent is present that will change color when that protein is bound to the solid surface. So very simple, easy to use, very specific for the HCG protein. Let's just look at our other two answer choices and the answer to being thorough choice. A a glutin nation test is a way of testing if a serum contains antibodies that will cause agglutination recall that agglutination is that clumping together of bacterial cells in the presence of an antibody as the antibody binds to it. But of course, we're not looking for the presence of bacteria in a pregnancy test. We're looking for the specific HCG protein. So we're only interested in the antibodies to that protein in that they let us know if HCG is present. So we're looking for the presence of a specific protein and therefore, an agglutination test is not an appropriate test. And then choice d precipitation test looks for the formation of these large complexes of antigens and antibodies that begin to precipitate out a solution. So in this test, you're looking for the formation of a precipitate as you have antibodies and antigens forming these large complexes. But this occurs when concentrations of both the antibody and antigen are high in a solution. So this is not appropriate for a pregnancy test where especially early in pregnancy, you're looking for very, very small amounts of HCG. So choice d not the correct technique for a pregnancy test. So the serological test utilized in a pregnancy test is choice C Eliza hope to see you in the next video.

Match each of the following tests in column A to its positive reaction in column B.
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Verified Solution

Key Concepts

Microbiological Tests

Positive Reaction Indicators

Test Matching

Match each of the following tests in column A to its positive reaction in column B.<IMAGE>

Verified Solution

Key Concepts

Microbiological Tests

Positive Reaction Indicators

Test Matching

Match each of the following tests in column A to its positive reaction in column B.
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