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Ch. 7 - Microbial Genetics
Chapter 7, Problem 7.2a

This bacteriophage DNA molecule has been warmed. Label the portions that likely have a higher ratio of GC base pairs and the portions that have a higher ratio of AT base pairs.

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1
Identify the regions of the bacteriophage DNA molecule that are more stable at higher temperatures.
Recall that GC base pairs have three hydrogen bonds, while AT base pairs have two hydrogen bonds.
Understand that regions with a higher GC content are more stable and less likely to denature at elevated temperatures.
Label the portions of the DNA molecule that remain intact or denature more slowly as having a higher GC content.
Label the portions of the DNA molecule that denature more quickly as having a higher AT content.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

GC and AT Base Pairing

In DNA, the bases adenine (A) and thymine (T) pair together, while guanine (G) and cytosine (C) pair together. The GC base pairs form three hydrogen bonds, making them more stable than AT pairs, which form only two. This stability affects the melting temperature of DNA, with regions rich in GC pairs typically having higher melting points compared to AT-rich regions.
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DNA Melting Temperature

The melting temperature (Tm) of DNA is the temperature at which half of the DNA strands are in the double-helix state and half are in the 'melted' single-strand state. Tm is influenced by the GC content; higher GC content results in a higher Tm due to the increased number of hydrogen bonds. Understanding Tm is crucial for predicting the behavior of DNA during processes like denaturation and hybridization.
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Bacteriophage Structure

Bacteriophages, or phages, are viruses that infect bacteria and consist of a nucleic acid core (DNA or RNA) surrounded by a protein coat. The structure of the phage can influence its stability and interaction with host cells. Recognizing the regions of the phage DNA that are rich in GC or AT pairs can provide insights into its stability and potential behavior during infection.
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Lytic & Lysogenic Bacteriophages