What would be the likely impact on the rate of a biochemical reaction catalyzed by an enzyme that functions optimally at room temperature (about 25°C) if a reducing agent like dithiothreitol (DTT) is introduced?

• A. The rate of the biochemical reaction will increase significantly.
• B. The rate of the biochemical reaction will decrease significantly.
• C. There will be no change in the rate of the biochemical reaction.
• D. The effect on the rate of the biochemical reaction cannot be determined.

Answer: (B)