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Ch. 9 - The Molecular Biology of Translation
Chapter 9, Problem 12

The diagram of a eukaryotic ribosome shown below contains several errors.

Redraw the diagram, and correct each error using the mRNA sequence shown. <>

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1
Identify the components of a eukaryotic ribosome, including the large and small subunits, and their respective rRNA and protein components.
Examine the mRNA sequence provided and determine the correct orientation and position of the mRNA in relation to the ribosome.
Ensure that the start codon (AUG) on the mRNA is correctly positioned in the P site of the ribosome, where translation begins.
Check the alignment of the tRNA molecules, ensuring that the anticodons are correctly paired with the codons on the mRNA sequence.
Verify that the A, P, and E sites of the ribosome are correctly labeled and that the process of translation is accurately depicted, including the movement of tRNA and mRNA through the ribosome.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Eukaryotic Ribosome Structure

Eukaryotic ribosomes are complex molecular machines composed of ribosomal RNA (rRNA) and proteins. They consist of two subunits: the large subunit (60S) and the small subunit (40S). These subunits come together during protein synthesis to translate mRNA into polypeptides, with the small subunit binding to the mRNA and the large subunit facilitating peptide bond formation.
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Ribosome Structure

mRNA Sequence and Codons

Messenger RNA (mRNA) is a single-stranded molecule that carries genetic information from DNA to the ribosome for protein synthesis. The mRNA sequence is read in sets of three nucleotides called codons, each of which corresponds to a specific amino acid. Understanding the correct codon sequence is essential for accurately translating the mRNA into a functional protein.
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Sequencing Difficulties

Translation Process

Translation is the process by which ribosomes synthesize proteins using the information encoded in mRNA. It involves three main stages: initiation, elongation, and termination. During initiation, the ribosome assembles around the mRNA; during elongation, amino acids are added one by one to the growing polypeptide chain; and during termination, the completed protein is released when a stop codon is reached.
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