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Ch. 12 - Regulation of Gene Expression in Bacteria and Bacteriophage
Chapter 12, Problem 4

Identify similarities and differences between an inducible operon and a repressible operon in terms of

the organization of structural genes of the operon.

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1
Understand that an operon is a cluster of genes under the control of a single promoter and is transcribed into a single mRNA strand.
Recognize that an inducible operon is typically off and requires an inducer molecule to activate transcription. A classic example is the lac operon in E. coli.
Identify that a repressible operon is usually on and can be turned off by a repressor molecule. The trp operon in E. coli is a well-known example.
Compare the organization of structural genes: In both types of operons, structural genes are organized in a sequence and are transcribed together, but the regulatory mechanisms differ.
Note that in inducible operons, the presence of an inducer prevents the repressor from binding to the operator, allowing transcription. In repressible operons, the presence of a corepressor enables the repressor to bind to the operator, blocking transcription.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Operon Structure

An operon is a cluster of genes under the control of a single promoter, allowing for coordinated expression. In prokaryotes, operons can be classified as inducible or repressible based on their regulatory mechanisms. The structural genes within an operon are transcribed together, producing a single mRNA that encodes multiple proteins, which often function in related pathways.
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Inducible Operon

An inducible operon is typically off but can be turned on in response to an inducer molecule. This type of operon, such as the lac operon, allows for the expression of genes involved in metabolizing specific substrates when they are present. The presence of the inducer leads to the inactivation of a repressor protein, enabling transcription of the structural genes.
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Repressible Operon

A repressible operon is usually active but can be turned off by a corepressor molecule. This mechanism is exemplified by the trp operon, which regulates the synthesis of tryptophan. When tryptophan levels are high, it binds to the repressor, activating it and inhibiting the transcription of the operon's structural genes, thus preventing unnecessary production.
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