Ch. 6 - Genetic Analysis and Mapping in Bacteria and Bacteriophages
All textbooksKlug 12th EditionCh. 6 - Genetic Analysis and Mapping in Bacteria and BacteriophagesProblem 11
Chapter 6, Problem 11
Explain the observations that led Zinder and Lederberg to conclude that the prototrophs recovered in their transduction experiments were not the result of F⁺ mediated conjugation.
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Textbook Question
Describe the origin of F' bacteria and merozygotes.
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In a transformation experiment, donor DNA was obtained from a prototroph bacterial strain (a⁺b⁺c⁺) and the recipient was a triple auxotroph (a⁻b⁻c⁻). What general conclusions can you draw about the linkage relationships among the three genes from the following transformant classes that were recovered?
a⁺ b⁻ c⁻ 180
a⁻ b⁺ c⁻ 150
a⁺ b⁺ c⁻ 210
a⁻ b⁻ c⁺ 179
a⁺ b⁻ c⁺ 2
a⁻ b⁺ c⁺ 1
a⁺ b⁺ c⁺ 3
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Describe the role of heteroduplex formation during transformation.
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Define plaque, lysogeny, and prophage.
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Textbook Question
Two theoretical genetic strains of a virus (a⁻b⁻c⁻ and a⁺b⁺c⁺) were used to simultaneously infect a culture of host bacteria. Of 10,000 plaques scored, the following genotypes were observed. Determine the genetic map of these three genes on the viral chromosome. Decide whether interference was positive or negative.
a⁺ b⁺ c⁺ 4100 a⁻ b⁺ c⁻ 160
a⁻ b⁻ c⁻ 3990 a⁺ b⁻ c⁺ 140
a⁺ b⁻ c⁻ 740 a⁻ b⁻ c⁺ 90
a⁻ b⁺ c⁺ 670 a⁺ b⁺ c⁻ 110
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Textbook Question
The bacteriophage genome consists of many genes encoding proteins that make up the head, collar, tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?
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