Chapter 19, Problem 10
Outline the roles played by restriction enzymes and vectors in cloning DNA.
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What are the advantages of using a restriction enzyme whose recognition site is relatively rare? When would you use such enzymes?
Does genetic analysis by ASO testing allow for detection of epigenetic changes that may contribute to a genetic disorder? Explain your answer.
In 1975, the Asilomar Conference on Recombinant DNA was organized by Paul Berg, a pioneer of recombinant DNA technology, at a conference center at Asilomar State Beach in California. Physicians, scientists, lawyers, ethicists, and others gathered to draft guidelines for safe applications of recombinant DNA technology. These general guidelines were adopted by the federal government and are still in practice today. Consider the implications of recombinant DNA as a new technology. What concerns might the scientific community have had then about recombinant DNA technology? Might those same concerns exist today?
Maternal blood tests for three pregnant women revealed they would be having boys, yet subsequent ultrasound images showed all three were pregnant with girls. In each case Y chromosome sequences in each mother's blood originated from transplanted organs they had received from men! This demonstrates one dramatic example of a limitation of genetic analysis of maternal blood samples. What kind of information could have been collected from each mother in advance of these tests to better inform physicians prior to performing each test?
In the context of recombinant DNA technology, of what use is a probe?