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Ch. 10 - DNA Structure and Analysis
Chapter 10, Problem 31

A primitive eukaryote was discovered that displayed a unique nucleic acid as its genetic material. Analysis provided the following information: A major hyperchromic shift is evident upon heating and monitoring UV absorption at 260 nm.

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Identify the type of nucleic acid based on the hyperchromic shift. A hyperchromic shift is an increase in UV absorption, typically observed when double-stranded nucleic acids are denatured into single strands.
Understand that the hyperchromic effect is characteristic of DNA, as the separation of strands during heating increases UV absorption due to the exposure of more nucleotide bases.
Consider the wavelength of 260 nm, which is the absorption peak for nucleic acids, particularly DNA and RNA, due to the presence of aromatic bases.
Evaluate the possibility of the nucleic acid being DNA or RNA. Since the hyperchromic shift is more pronounced in DNA due to its double-stranded nature, this suggests the genetic material is likely DNA.
Conclude that the unique nucleic acid in the primitive eukaryote is likely DNA, as evidenced by the hyperchromic shift upon heating, indicating strand separation typical of DNA.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Hyperchromic Effect

The hyperchromic effect refers to the increase in absorbance of UV light by nucleic acids when they are denatured or heated. This phenomenon occurs because the base pairs in double-stranded DNA or RNA are disrupted, allowing more UV light to be absorbed. Monitoring this shift at 260 nm is crucial for assessing the stability and integrity of nucleic acids.
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Nucleic Acids

Nucleic acids, including DNA and RNA, are biopolymers essential for all known forms of life. They serve as the genetic material, encoding the information necessary for the growth, development, and reproduction of organisms. Understanding the structure and function of nucleic acids is fundamental in genetics, particularly when analyzing genetic material in primitive organisms.
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Eukaryotic Cells

Eukaryotic cells are complex cells characterized by the presence of a nucleus and membrane-bound organelles. Unlike prokaryotes, eukaryotes can be unicellular or multicellular and include organisms such as plants, animals, and fungi. The discovery of a primitive eukaryote with unique nucleic acids suggests evolutionary significance and potential insights into the origins of genetic material.
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Related Practice
Textbook Question
Consider the drawing of a dinucleotide below. Is the arrow closest to the 5' or the 3' end?
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Textbook Question
Consider the drawing of a dinucleotide below. Suppose that the molecule was cleaved with the enzyme spleen phosphodiesterase, which breaks the covalent bond connecting the phosphate to C-5'. After cleavage, to which nucleoside is the phosphate now attached (A or T)?
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Textbook Question
A primitive eukaryote was discovered that displayed a unique nucleic acid as its genetic material. Analysis provided the following information: The general X-ray diffraction pattern is similar to that of DNA, but with somewhat different dimensions and more irregularity.
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Textbook Question
A primitive eukaryote was discovered that displayed a unique nucleic acid as its genetic material. Analysis provided the following information: Base-composition analysis reveals four bases in the following proportions: Adenine = 8% Guanine = 37% Xanthine = 37% Hypoxanthine = 18%
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Textbook Question
A primitive eukaryote was discovered that displayed a unique nucleic acid as its genetic material. Analysis provided the following information: About 75 percent of the sugars are deoxyribose, while 25 percent are ribose. Postulate a model for the structure of this molecule that is consistent with the foregoing observations.
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Textbook Question
Newsdate: March 1, 2030. A unique creature has been discovered during exploration of outer space. Recently, its genetic material has been isolated and analyzed. This material is similar in some ways to DNA in its chemical makeup. It contains in abundance the 4-carbon sugar erythrose and a molar equivalent of phosphate groups. In addition, it contains six nitrogenous bases: adenine (A), guanine (G), thymine (T), cytosine (C), hypoxanthine (H), and xanthine (X). These bases exist in the following relative proportions: A =T = H and C = G = X X-ray diffraction studies have established a regularity in the molecule and a constant diameter of about 30 Å. Together, these data have suggested a model for the structure of this molecule. Propose a general model of this molecule. Describe it briefly.
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