A wild-type culture of haploid yeast is exposed to ethyl methanes...

Question

A wild-type culture of haploid yeast is exposed to ethyl methanesulfonate (EMS). Yeast cells are plated on a complete medium, and 6 colonies (colonies numbered 1 to 6) are transferred to a new complete medium plate for further study. Four replica plates are made from the complete medium plate to plates containing minimal medium or minimal medium plus one amino acid4 (replica plates numbered 1 to 4) with the following results:

Are there any colonies for which genotype information cannot be determined? If so, which colony or colonies?

Accepted Answer

Hey, everyone. Let's take a look at this question. Together. E coli bacterial culture undergoes exposure to ultraviolet radiation. Subsequently, the bacterial cells are cultivated on a complete medium and eight individual colonies are chosen for further analysis. The complete medium plate is used to create four replica plates which contain minimal medium or minimal medium added with one antibiotic. The results obtained are as follows. And here we have a table showing the eight different colonies and the effect on those eight different colonies. Can you identify the colony or colonies that exhibit resistance to all the tested antibiotics? And we have four different answer choices containing two different colony combinations that we have to determine of the following combinations which two are resistant to all the tested antibiotics. So from the table of the eight different colonies, by looking at the effect on each colony, we are able to determine that colony one and colony eight are resistant to all the tested antibiotics, which means that the colonies that exhibit resistance to all the tested antibiotics are colony one and eight, which is answer choice. A the correct answer since colonies two through seven only exhibit resistance to some and not all of the tested antibiotics and only colonies. One and eight exhibit that resistance to all of the tested antibiotics, which is answer choice. A so it is the correct answer. I hope you found this video to be helpful. Thank you and goodbye.

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