Matthew Meselson and Franklin Stahl demonstrated that DNA replica...

Question

Matthew Meselson and Franklin Stahl demonstrated that DNA replication is semiconservative in bacteria. Briefly outline their experiment and its results for two DNA replication cycles, and identify how the alternative models of DNA replication were excluded by the data.

Accepted Answer

Hi, everybody. Let's look at our next question. It says the enzyme that is responsible for the formation of Kleo fragments is and our answer choices are a DNA polymerase. Three B R N A polymerase C, DNA polymerase, one or D, none of these. So let's recall what the glen fragment is and it is a large portion of DNA Polymerase one. So that will be choice. C We know that DNA Polymerase one is responsible for its formation. Since it's part of that protein, It contains two out of three domains and DNA plum rse one is an important proof reading enzyme. And so as a result, it has polymerase activity In the five prime to three prime direction. And then it has exonuclease activity In both directions, five prime to three prime and three prime to five prime as part of its proof reading activity to be able to remove erroneous bases. And the clean out fragment has the five prime to three prime exonuclease activity remove. So this is removed in the fragment. So that's the domain that's missing the domain that codes for the five prime to three prime exonuclease activity. And it has many uses in molecular biology, which is why you produce that. And it can be used to fill in uh sticky ends with its polymerase activity and trim off dangling uh three prime ends and it can be used to create primers. So that's why you actually would make this particular protein there. So we know that our other answer choices. It's not none of these choice. D isn't our answer since we have a correct answer here And it's not DNA Polymerase three or are a polymer the we want for the enzyme responsible for the formation of pleo fragments is choice C DNA polymerase one. See you in the next video.

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Matthew Meselson and Franklin Stahl demonstrated that DNA replication is semiconservative in bacteria. Briefly outline their experiment and its results for two DNA replication cycles, and identify how the alternative models of DNA replication were excluded by the data.