5. Genetics of Bacteria and Viruses
Bacterial Conjugation
2: minutes
Problem 23b
Textbook Question
Textbook QuestionSynthesis of the amino acid histidine is a multistep anabolic pathway that uses the products of 13 genes (hisA to hisM) in E. coli. Two independently isolated E. coli mutants, designated his1⁻ and his2⁻ are studied in a conjugation experiment. A his⁺ F' donor strain that carries a copy of the hisJ gene on the plasmid is mated with a his1⁻ recipient strain in Experiment 1 and with a his2⁻ recipient in Experiment 2. The exconjugants are grown on plates lacking histidine. Growth is observed among the exconjugants of Experiment 2 but not among those of Experiment 1.
Why is growth observed in Experiment 2 but not in Experiment 1?
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