The flow of genetic information from DNA to protein is mediated b...

Question

The flow of genetic information from DNA to protein is mediated by messenger RNA. If you introduce short DNA strands (called antisense oligonucleotides) that are complementary to mRNAs, hydrogen bonding may occur and 'label' the DNA/RNA hybrid for ribonuclease-H degradation of the RNA. One study [Lloyd et al. (2001). Nucl. Acids Res. 29:3664–3673] compared the effect of different-length antisense oligonucleotides upon ribonuclease-H–mediated degradation of tumor necrosis factor (TNFα) mRNA. TNFα exhibits antitumor and pro-inflammatory activities. The following graph indicates the efficacy of various-sized antisense oligonucleotides in causing ribonuclease-H cleavage.

What general conclusion can be drawn from the graph?

Accepted Answer

Welcome back. Here's our next problem. The direction of polyp peptide elongation during protein synthesis is we know so our answer choices are for A. And B. We have five prime, three prime and three prime five prime C. And D. We have choices of N. Two C. Or C. Two N. So let's think about a process of translation. For my kind of sad little diagram here, here is our M. R. N. A. And here's our ribs um coming along and we see our ribs um walks along the M. RNA in a five prime three prime direction as it's going along. We have T. R. N. A. S. A. Weird looking P. R. N. A. Bringing in the next amino acid in the transcript here. And we see here's our growing protein chain exiting the ribs um as is being synthesized. So when we're talking about the direction of polyp peptide elongation were saying as we add new amino acids, what direction is the polyp peptide chain being added to? And so that we're not going to look at the five prime, 23 prime or three prime five prime. That's the direction the ribs um is moving along the M. R. N. A. So that's not going to be what we're looking for. We want to look at the ends of the polyp peptide chain and there's an end terminus and a C terminus where they mean a group and the car boxing group. So let's look at our growing polyp peptide chain here and we see that the end terminus is what we started with. So we know that our direction of polyp peptide elongation must be N two C. So here's our answer here, Choice C. And we can eliminate choice D. So again, the direction of polyp peptide elongation during protein synthesis is Choice C and C. C. In the next video.

Key Concepts

Genetic Information Flow

Antisense Oligonucleotides

Ribonuclease H Activity

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