Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
4. Genetic Mapping and Linkage
Trihybrid Cross
Problem 26c
Textbook Question
The following progeny are obtained from a test cross of a trihybrid wild-type plant to a plant with the recessive phenotypes compound leaves (c), intercalary leaflets (i), and green fruits (g). (Traits not listed are wild type.) The test-cross progeny are as follows:
Phenotype Number
Compound leaves 324
Compound leaves, intercalary leaflets 32
Compound leaves, green fruits 5
Compound leaves, intercalary leaflets, green fruits 51
Intercalary leaflets 3
Intercalary leaflets, green fruits 309
Green fruits 42
Wild type 49
815
Determine the order of the three genes, and construct a genetic map that identifies the correct order and the alleles carried on each chromosome in the trihybrid parental plant.
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1
Identify the parental and recombinant phenotypes by looking at the numbers of progeny. The most frequent phenotypes are the parental types, while the least frequent are the double crossovers.
Determine the gene order by comparing the parental and double crossover phenotypes. The gene that differs between these two sets is the one in the middle.
Calculate the recombination frequencies between each pair of genes by adding the number of single and double crossover progeny for each pair and dividing by the total number of progeny.
Convert the recombination frequencies into map distances (in centimorgans, cM) using the formula: 1% recombination = 1 cM.
Construct the genetic map by placing the genes in the determined order and marking the distances between them based on the calculated map distances.
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Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Gene Mapping
Gene mapping is the process of determining the relative positions of genes on a chromosome. It involves analyzing the frequency of recombination between different genes during meiosis. By examining the offspring of test crosses, researchers can infer the order of genes based on how often they are inherited together, which helps in constructing a genetic map.
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Mapping Genes
Test Cross
A test cross is a breeding experiment used to determine the genotype of an individual with a dominant phenotype. This is done by crossing the individual with a homozygous recessive individual. The phenotypic ratios of the offspring provide insights into the alleles present in the parent, allowing for the identification of dominant and recessive traits.
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Trihybrid Cross
Recombination Frequency
Recombination frequency refers to the likelihood that two genes will be separated during meiosis due to crossing over. It is calculated by dividing the number of recombinant offspring by the total number of offspring. This frequency is crucial for gene mapping, as it helps to estimate the distance between genes on a chromosome, with lower frequencies indicating closer proximity.
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Recombination after Single Strand Breaks
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