17. Mutation, Repair, and Recombination
Types of Mutations
1:30 minutes
Problem 11b
Textbook Question
Textbook QuestionTwo different mutations are identified in a haploid strain of yeast. The first prevents the synthesis of adenine by a nonsense mutation of the ade-1 gene. In this mutation, a base-pair substitution changes a tryptophan codon (UGG) to a stop codon (UGA). The second affects one of several duplicate tRNA genes. This base-pair substitution mutation changes the anticodon sequence of a tRNAᵀʳᵖ from
3′−ACC−5′ to 3′−ACU−5′
Assuming there are no other mutations in the genome, will this double-mutant yeast strain be able to grow on minimal medium? If growth will occur, characterize the nature of growth relative to wild type.
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