The two gels illustrated contain dideoxynucleotide DNA-s...

Question

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.

What is the cause of the mutation?

Accepted Answer

Hi, everybody. Let's look at our next problem. It says in New Caros, many mutations occur in the non coding regions. When these mutations do not affect gene products or gene expression, they are considered as joint a choice. A point mutations. B nonsense mutations, C neutral mutations or D missense mutations. When we have a mutation in the non coding region that doesn't affect gene products or gene expression. That second phrase is important because just because it occurs in a non coding region doesn't mean it won't have an effect on gene products or the gene expression. If it, for instance, affects regulatory regions because some of the non coding regions are involved in the regulation of gene expression, then that is something separate. That's not what we're talking about here. So we're talking about a mutation in a non coding region. So you know, those large noncoding areas that seemed to do nothing. And if we have a mutation in that region that does not affect the gene product or the gene expression, what are they? And the answer would be choice c neutral mutations, they're neutral, no effect on the organism. So let's look at our other answer choices to understand why they are not correct choice. A point mutation would be a mutation to a single base pair. Note that this could occur in a non coding region if you just have a single base changed. But our question specifically says, what is the specific name for a mutation that doesn't affect the gene in the non coding region? So point mutation just is a term for a mutation to a single base. So that's not our correct answer. Choice. B nonsense mutation is when you have a mutation to a codon that changes or to a base, excuse me, that changes a codon to a nonsense code on or a stop codon. So that's why it's a nonsense mutation but not what we're looking for. So not our correct answer. And then finally, choice D missense muta mutations that is when you have a mutation that changes a code on to code for a different amino acid. So you've changed 12 or three base pairs and that's resulted in a different amino acid replacing the one originally in the gene. But again, that's not what we're looking for here because we're looking for specifically a mutation to the non coding region. So choice D is not correct. So mutations in the non coding region that do not affect gene products or gene expression. Our choice C neutral mutations since they have no effect on the organisms phenotype. See you in the next video

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.

What is the cause of the mutation?

Key Concepts

Dideoxynucleotide Sequencing

Codons and Start Codon

Mutations and Their Effects

Watch next

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.What is the cause of the mutation?

Key Concepts

Dideoxynucleotide Sequencing

Codons and Start Codon

Mutations and Their Effects

Watch next

The two gels illustrated contain dideoxynucleotide DNA-sequencing information for a wild-type segment and mutant segment of DNA corresponding to the N-terminal end of a protein. The start codon and the next five codons are sequenced.

What is the cause of the mutation?