The CBF genes of Arabidopsis are induced by exposure of the plant...

Question

The CBF genes of Arabidopsis are induced by exposure of the plants to low temperature.

How would you examine the temporal and spatial patterns of expression after induction by low temperature?

Accepted Answer

Hello, everyone and welcome to today's video. So which high throughput technique can provide a comprehensive assessment of gene expression changes in response to low temperatures in these Arabidopsis plants. As answer choice A, we have PC R as answer choice B we have western blood as answer choice C, we have ELISA and as answer choice D we have R N A sequencing. So let's go over each of our answer, each of our answer choices. Keeping in mind that if we want to measure gene expression, the best marker for these is going to be M R and if a gene is expressed, it is going to be producing M R N A. So if we are able to measure these M R N changes, we're going to be able to measure gene expression. So let's go over each of our answer choices as answer choice A, we have PC R which is preliminary chain reaction. So if you recall from previous videos, this is going to be used to replicate Danny. So it is not really going to be involved in M R N A measuring. So we're going to cancel it out, then we have both western blotting and ELISA. And the reason why we're taking them together is because these two techniques are used to measure specific proteins. They're not going to be used to measure M R N A changes. So we're going to cancel both of them up. And finally, we have R N A sequencing. And if you recall from previous videos, this is going to allow for the identification of differentially expressed genes and the quantification of M R N A levels inside of a cell. So if we do this over time, we can detect certain changes to the M R N levels within of the cell and measure gene expression. Therefore, answer choice D R N A sequencing between parentheses R N A is going to be the correct answer for this question. I really hope this helped you and I hope to see you on the next video.

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The CBF genes of Arabidopsis are induced by exposure of the plants to low temperature. How would you examine the temporal and spatial patterns of expression after induction by low temperature?