Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
5. Genetics of Bacteria and Viruses
Bacterial Conjugation
1:24 minutes
Problem 24b
Textbook Question
Textbook QuestionAn Hfr strain is used to map three genes in an interrupted mating experiment. The cross is Hfr/a⁺b⁺c⁺ rif x F⁻/a⁻b⁻c⁻ rif^T (No map order is implied in the listing of the alleles; rif^T is resistance to the antibiotic rifampicin.) The a⁺ gene is required for the biosynthesis of nutrient A, the b⁺ gene for nutrient B, and c⁺ for nutrient C. The minus alleles are auxotrophs for these nutrients. The cross is initiated at time = 0 and at various times, the mating mixture is plated on three types of medium. Each plate contains minimal medium (MM) plus rifampicin plus specific supplements that are indicated in the following table. (The results for each time interval are shown as the number of colonies growing on each plate.) Time of Interruption _ 5 min 10 min 15 min 20 min Nutrients A and B 0 0 4 21 Nutrients B and C 0 5 23 40 Nutrients A and C 4 25 60 82 Based on these data, determine the approximate location on the chromosome of the a, b, and c genes relative to one another and to the F factor.
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