Skip to main content
Ch. 20 - The Molecular Revolution: Biotechnology, Genomics, and New Frontiers

Chapter 19, Problem 9

Revolutionaries executed Nicholas II, the last czar of Russia, along with his wife and five children, the family physician, and about a dozen servants. Many decades later, a grave said to hold the remains of the royal family was discovered. Biologists were asked to analyze DNA from the bodies. If the remains of the family were in this grave, predict how similar the DNA fingerprints would be between the parents, the children, and the unrelated individuals in the grave.

Verified Solution
Video duration:
3m
This video solution was recommended by our tutors as helpful for the problem above.
Was this helpful?

Video transcript

Hi everyone. This is our next question. Which one of the following techniques is helpful in identifying the dead bodies of families buried in a grave. When we talk about identifying we can think of you might use fingerprinting and that will bring to mind D. N. A. Fingerprinting. And this involves looking for for unique patterns in specific regions of the genome that very among individuals. And because your DNA is inherited from her parents this regional variation can be used to show relatedness. So the unique patterns can identify individuals. And then because those patterns are inherited from two parents they can be used to help show relatedness among different individuals. So if we're talking about identifying dead bodies we can use extract D. N. A. And use the techniques of DNA fingerprinting to identify them. So that's that would be Choice C. DNA fingerprinting Choice A. Is D. N. A. Sequencing. Um This theoretically could be used because you'd be looking at the actual D. N. A. Sequence of the individuals. But this would produce huge amounts of data. It wouldn't really be practical to look at the entire genome of the bodies who are trying to identify better. To use the fingerprinting technique in which you zoom in on those regions that tend to vary from individual to individual. So that's why A. Is not our answer Choice B recombinant DNA technology. Well that doesn't have anything to do with identifying individuals that involves um putting jeans uh in from something that you're studying into yeast or bacterial cells to produce proteins Or look at these genes in your studies. So or if you want to produce a protein and make you turn your bacteria yeast into a little factory to make a specific protein that they don't usually produce. So that's why Choice B is not our answer. And then Joyce de cloning. Cloning obviously refers to generating individuals that are identical genetic copies of the source individual. So that's definitely not what we're talking about here. So which of the following techniques is helpful in identifying the dead bodies of families buried in a grave. Choice C. D. N. A fingerprinting. See you in the next video.
Related Practice
Open Question

Explain what qualifies the gene therapy method to cure sickle cell disease as an ex vivo approach.

5
views
Textbook Question

After finding a gene that causes a disease, researchers often introduce the defective allele into mice to create an animal model of the disease. Why are these models valuable? a. They allow the testing of potential drug therapies without endangering human patients. b. They allow the sequencing of the mutant allele. c. They allow the production of large quantities of the defective gene product, usually a protein. d. They allow the study of how the gene was transmitted from parents to offspring.

413
views
Textbook Question

A friend who works in a research lab performed a GWAS and discovered a tight association between a SNP allele and the disease she is studying. She concluded that the SNP allele must be the mutation that causes the disease. Explain why she is likely to be wrong.

237
views
Textbook Question

Potato blight causes potato plants to shrivel and rot. The disease is caused by the pathogen Phytophthora infestans, infamous for its role in Ireland's Great Potato Famine in the mid-1840s. The disease can devastate crops during wet weather, sometimes leading to total crop loss. Researchers aim to use recombinant DNA methods to transfer blight resistance genes from resistant varieties into susceptible varieties of potato. Explain how restriction endonucleases and DNA ligase could be used to insert a potato blight resistance gene into a plasmid.

396
views
Textbook Question

Potato blight causes potato plants to shrivel and rot. The disease is caused by the pathogen Phytophthora infestans, infamous for its role in Ireland's Great Potato Famine in the mid-1840s. The disease can devastate crops during wet weather, sometimes leading to total crop loss. Researchers aim to use recombinant DNA methods to transfer blight resistance genes from resistant varieties into susceptible varieties of potato. Transgenic plants usually contain genes of bacterial plasmid origin. In a recent study, researchers designed a strategy that avoided using any plasmid genes. They transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. Next, to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences, they performed PCR using primers specific for the plasmid. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA. Based on the gel analysis of PCR products shown below, which plants contain only the potato gene? Explain your answer.

325
views
Textbook Question

If the sequence of DNA in Question 12 were amplified using 25 PCR cycles, then the amount of this DNA would be predicted to increase by          -fold.

316
views