Chapter 20, Problem 7
Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of a. post-transcriptional processing. b. post-translational processing. c. nucleic acid hybridization. d. restriction fragment ligation.
Video transcript
A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird). To compare a specific region of the DNA from a sample with DNA from living birds, which of the following would be most useful for increasing the amount of dodo DNA available for testing? a. SNP analysis b. polymerase chain reaction (PCR) c. electroporation d. gel electrophoresis
Which of the following is not true of cDNA produced using human brain tissue as the starting material? a. It can be amplified by the polymerase chain reaction. b. It was produced from pre-mRNA using reverse transcriptase. c. It can be labeled and used as a probe to detect genes expressed in the brain. d. It lacks the introns of the pre-mRNA.
Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme? a. AAGG TTCC b. GGCC CCGG c. ACCA TGGT d. AAAA TTTT