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Ch. 12 - The Cell Cycle
Chapter 12, Problem 9

VISUAL SKILLS The light micrograph shows dividing cells near the tip of an onion root. Identify a cell in each of the following stages: prophase, prometaphase, metaphase, anaphase, and telophase. Describe the major events occurring at each stage.

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Identify each stage by examining the arrangement and appearance of chromosomes and the spindle fibers. In prophase, look for cells where chromatin condenses into visible chromosomes and the nuclear envelope begins to break down. Spindle fibers start to form.
In prometaphase, identify cells where the nuclear envelope is completely dissolved, and chromosomes are more condensed. Spindle fibers attach to the kinetochores of chromosomes.
For metaphase, find cells where chromosomes are aligned at the metaphase plate (equatorial plane) of the cell, with spindle fibers attached to the kinetochores of the chromosomes.
In anaphase, look for cells where sister chromatids are separating and moving towards opposite poles of the cell, pulled by the spindle fibers.
For telophase, identify cells where chromosomes arrive at the poles and begin to decondense, nuclear envelopes re-form around each set of chromosomes, and the spindle fibers disassemble. This stage is followed by cytokinesis, where the cell divides into two.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Mitosis

Mitosis is the process of cell division that results in two genetically identical daughter cells from a single parent cell. It consists of several stages: prophase, prometaphase, metaphase, anaphase, and telophase, each characterized by specific events that ensure the accurate distribution of chromosomes. Understanding mitosis is crucial for identifying the stages in dividing cells, such as those in an onion root tip.
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Chromosome Structure

Chromosomes are structures within cells that contain DNA and proteins, and they play a vital role during cell division. Each chromosome is made up of two sister chromatids joined at a region called the centromere. During mitosis, the organization and alignment of chromosomes are critical for their proper segregation, making it essential to recognize their structure when identifying stages of mitosis.
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Cell Cycle Regulation

The cell cycle is regulated by a series of checkpoints that ensure the cell is ready to proceed to the next phase of division. These checkpoints monitor DNA integrity, chromosome alignment, and cell size, preventing errors that could lead to abnormal cell division. Understanding cell cycle regulation helps explain the importance of each mitotic stage and the consequences of errors during these processes.
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