Introduction to DNA-Based Technology - Video Tutorials & Practice Problems
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Introduction to DNA-Based Technology
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2m
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This video, we're going to begin our introduction to DNA based technology. And so DNA based technology is really just a term that's used to describe the techniques used to manipulate DNA or change the DNA sequence and to study gene expression. And so there are many different reasons to study DNA, and some of those reasons are listed here in parentheses, such as developing vaccines, genetically modifying plants, and tracking inheritance within families. And so down below in our example, we're showing you some images here of some of the many reasons why researchers use DNA based technologies to study gene expression. And so over here on the far left hand side, this is of course showing you the development of vaccines, vaccines, such as for example the COVID nineteen vaccine. Now DNA based technologies can also be used to genetically modify plants. To give plants specific characteristics, such as maybe modifying the DNA of a plant so that it has more resistance to pesticides. And so in this image down below over here, notice that this plant over here is having a problem with these pests, and he's got this little whacker here to try to get rid of them. Whereas this plant over here is not having that same problem, and that's because this plant has been genetically modified to have specific characteristics that allows it to resist pesticides. And so he's saying, you gotta have the right genes, buddy, in order to avoid this pest problem. And then last but not least over here, another of many, reasons to study DNA is to track inheritance patterns within families. And so down below, what we're showing you here is just an image of a little pedigree to show you that DNA based technologies can be really really important for tracking inheritance patterns. And so this is just some of the very many reasons why it's so important to study, DNA and to use DNA based technology. And so as we move forward in our course, we're going to talk about a lot of different, technologies. And so this here concludes our brief introduction to DNA based technology and I'll see you all in our next video.
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Map of DNA-Based Technology Lesson
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3m
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In this video, we're going to introduce our map of the lesson on DNA based technologies, which is down below right here. And so this image really is and can be used like a map to help guide you as we move forward through our lesson on DNA based technologies. And so notice at the very top we have our DNA based technologies and we've got these branches, and the way that this map is going to work is we are always going to be following the left most branches first and explore them to their end. So we'll start off over here exploring all of this. Then once we finish exploring all of that, we'll zoom out and explore the the next leftmost branch. Then we'll continue on in this pattern as you see like this. And last but not least, we'll cover this last region over here. And so what we're going to do first as we cover DNA based technologies is we are going to talk about DNA cloning, which you can see over here. And we've got DNA cloning lesson broken up into 2 parts. The first part is creating the recombinant DNA molecule, which involves using restriction enzymes to cut the DNA, which pretty much act like little tiny molecular scissors. We'll talk about that more as we move forward. And then, it also involves the ligation enzymes that are going to help paste the DNA. And so you can see here we have some DNA and we are going to cut and paste to create a recombinant DNA. And then the second step is going to be the transform that recombinant DNA into bacteria. And again, we're gonna talk all about this in more detail as we move forward in our course. Then after we talk about DNA cloning, we'll talk about the next branch here, which is going to be polymerase chain reaction, which is abbreviated as PCR, and we'll talk about the steps of PCR, which includes denaturation, annealing, and extension. After that, we'll move on to talk about DNA based technologies that are used for the separation of DNA samples. And this includes gel electrophoresis, southern blotting, and DNA finger printing. And then after we talk about the separation of DNA samples, we'll move on to the final section here of our map of the lesson, and that is going to be talking about DNA sequencing. And so we're gonna talk specifically about dideoxy sequencing And that's going to involve using chain termination PCR, and also determining the DNA sequence. And so this is really the map of our entire lesson on DNA based technologies. And again, you can use this map as you move forward as a guide to help you make predictions about what we're going to talk about next and to help make sure that you know where we are within our lesson. And so this here concludes our introduction to our map of DNA based technology lesson, and, I'll see you all in our next video.