Southern Blotting definitions Flashcards

The process of combining two complementary single-stranded DNA molecules to form a double-stranded molecule, often used to detect specific DNA sequences.

A segment of DNA that encodes functional products, typically proteins, and determines hereditary traits.

A single-stranded DNA molecule used in hybridization reactions to detect specific genes in DNA fragments often labeled for visualization in techniques like Southern blotting.

A technique that uses a labeled DNA probe to detect specific DNA sequences in a sample after gel electrophoresis and transfer to a membrane.

A technique that separates DNA fragments by size using an electric field applied to a gel matrix.

A scientist who studies genes, genetic variation, and heredity in organisms, often using techniques like Southern blotting to detect specific DNA sequences.

A medium used to transfer DNA samples for hybridization reactions, allowing detection of specific genes via labeled probes.

Marked with radioactive isotopes to detect specific DNA sequences in hybridization reactions.

A specific DNA sequence targeted by a probe in hybridization reactions to determine its presence in a DNA sample.