11:45The Principle of Southern Blotting and Northern Blotting, Blotting Techniques, The Full MechanismBiomedical and Biological Sciences616views
Multiple ChoiceA southern blotting technique is used to detect a specific ______ sequence from a blood or tissue sample.1603views
Multiple ChoiceA _____ is a single-stranded DNA molecule used in hybridization reactions to detect the presence of a particular gene in an assortment of DNA fragments.(Hybridization Reactions: reactions that combine two complementary single-stranded DNA molecules)792views
Multiple ChoiceA geneticist wants to see if her patient has Gene X. The geneticist takes a blood sample from her patient and prepares a southern blot. How will the geneticist know if her patient possesses Gene X?682views
Open QuestionPlace the following steps of Southern Blotting in the correct order.a) ______: Filter paper is incubated with the labeled DNA probe which anneals to the ssDNA fragments.b) ______: Analyze gel to determine the presence of the DNA sequence of interest.c) ______: Separate DNA fragments by size using gel electrophoresis.d) ______: Denature DNA by soaking gel in a basic solution.e) ______: Fragment unknown DNA sample(s) using restriction enzymes.879views7rank
Multiple ChoiceBy analyzing the Southern Blot results below, which of the samples contains the gene of interest?1234views
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