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Southern Blotting

Southern Blotting

18. Biotechnology / Southern Blotting / Problem 2

What is the purpose of soaking the gel derived from the initial step of Southern blotting in a denaturing buffer?

ANSWERS OPTIONS

A

To convert the double-stranded DNA into a single-stranded DNA.

B

To identify the genes associated with a DNA fragment.

C

To deactivate DNA synthesis.

D

To degrade the unnecessary DNA fragments.

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