Microbiology: A Laboratory Manual, 12th edition

Part 1 Basic Laboratory Techniques for Isolation, Cultivation, and Cultural

• Experiment 1: Effectiveness of Hand Washing
• Experiment 2: Culture Transfer Techniques
• Experiment 3: Techniques for Isolation of Pure Cultures
• Experiment 4: Cultural Characteristics of Microorganisms

Part 2 Microscopy

• Experiment 5: Microscopic Examination of Stained Cell Preparations
• Experiment 6:Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount

Part 3 Bacterial Staining

• Experiment 7: Preparation of Bacterial Smears
• Experiment 8: Simple Staining
• Experiment 9: Negative Staining
• Experiment 10:Gram Stain
• Experiment 11: Acid-Fast Stain
• Experiment 12: Differential Staining for Visualization of Bacterial Cell Structures

Part 4 Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations

• Experiment 13:Nutritional Requirements: Media for the Routine Cultivation of Bacteria
• Experiment 14: Use of Differential, Selective, and Enriched Media
• Experiment 15: Physical Factors: Temperature
• Experiment 16: Physical Factors: pH of the Extracellular Environment
• Experiment 17: Physical Factors: Atmospheric Oxygen Requirements
• Experiment 18: Techniques for the Cultivation of Anaerobic Microorganisms
• Experiment 19: Serial Dilution–Agar Plate Procedure to Quantitate Viable Cells
• Experiment 20: The Bacterial Growth Curve

Part 5 Biochemical Activities of Microorganisms

• Experiment 21: Extracellular Enzymatic Activities of Microorganisms
• Experiment 22: Carbohydrate Fermentation
• Experiment 23: Triple Sugar–Iron Agar Test
• Experiment 24: IMViC Test
• Experiment 25: Hydrogen Sulfide Test
• Experiment 26: Urease Test
• Experiment 27: Litmus–Milk Reactions
• Experiment 28: Nitrate Reduction Test
• Experiment 29: Catalase Test
• Experiment 30: Oxidase Test
• Experiment 31: Utilization of Amino Acids
• Experiment 32: Genus Identification of Unknown Bacterial Cultures

Part 6 The Protozoa

• Experiment 33: Free-Living Protozoa
• Experiment 34: Parasitic Protozoa

Part 7 The Fungi

• Experiment 35: Cultivation and Morphology of Molds
• Experiment 36: Isolation of a Soil Fungal Species
• Experiment 37:Morphology, Cultural Characteristics and Reproduction

Part 8 The Viruses

• Experiment 38: Cultivation and Enumeration of Bacteriophages
• Experiment 39: Isolation of Coliphages from Raw Sewage
• Experiment 40: Propagation of Isolated Bacteriophage Cultures

Part 9 Physical and Chemical Agents for the Control of Microbial Growth

• Experiment 41: Physical Agents of Control: Moist Heat
• Experiment 42: Chemical Agents of Control: Chemotherapeutic Agents
• Experiment 43: Determination of Penicillin Activity in the Presence and Absence of Penicillinase
• Experiment 44: Chemical Agents of Control: Disinfectants and Antiseptics

Part 10 Microbiology of Food

• Experiment 45: Microbiological Analysis of Food Products: Bacterial Count
• Experiment 46: Isolation of Salmonella from Raw Meat
• Experiment 47: Microbial Fermentation

PART 11 Microbiology of Water

• Experiment 48: Standard Qualitative Analysis of Water
• Experiment 49: Quantitative Analysis of Water: Membrane Filter Method

PART 12 Microbiology of Soil

• Experiment 50: Microbial Populations in Soil: Enumeration
• Experiment 51: Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates
• Experiment 52: Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique

PART 13 Bacterial Genetics

• Experiment 53: Enzyme Induction
• Experiment 54: Bacterial Conjugation
• Experiment 55: Isolation of a Streptomycin-Resistant Mutant
• Experiment 56: The Ames Test: A Bacterial Test System for Chemical Carcinogenicity
• Experiment 57: Utilization of Bacterial Plasmids
• Experiment 58: Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA

PART 14 Medical Microbiology

• Experiment 59: Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries
• Experiment 60: Normal Microbial Flora of the Throat and Skin
• Experiment 61: Identification of Human Staphylococcal Pathogens
• Experiment 62: Identification of Human Streptococcal Pathogens
• Experiment 63: Identification of Streptococcus pneumoniae
• Experiment 64: Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems
• Experiment 65: Isolation and Presumptive Identification of Campylobacter
• Experiment 66: Microbiological Analysis of Urine Specimens
• Experiment 67: Microbiological Analysis of Blood Specimens
• Experiment 68: Species Identification of Unknown Bacterial Cultures

PART 15 Immunology

• Experiment 69: Precipitin Reaction: The Ring Test
• Experiment 70: Agglutination Reaction: The Febrile Antibody Test
• Experiment 71: Enzyme-Linked Immunosorbent Assay
• Experiment 72: Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures